{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T12:14:30Z","timestamp":1762431270583,"version":"build-2065373602"},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,7,6]],"date-time":"2017-07-06T00:00:00Z","timestamp":1499299200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dental Traumatology"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background\/Aims<\/jats:title><jats:p>An easily available tooth storage medium is required to preserve a tooth after avulsion. Milk and Hank's balanced salt solution (<jats:styled-content style=\"fixed-case\">HBSS<\/jats:styled-content>) are recommended as tooth storage media, and egg white is also reported to be comparable with milk. The aim of this study was to histologically and immunohistochemically evaluate the effect of different tooth storage media on the periodontal ligament (<jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>) of extracted teeth.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>This experiment used <jats:styled-content style=\"fixed-case\">HBSS<\/jats:styled-content>, milk, and egg white as tooth storage media. A total of ninety\u2010six 6\u2010week\u2010old male Sprague\u2010Dawley rats were used in these experiments. In each experiment, six rats were used for each medium and for the control group. Extracted rat molar teeth were immersed in these three different storage media for 1\u00a0hour. In each medium, six samples (n=18) were fixed immediately, and the remaining samples (n=54) were subcutaneously transplanted. In the control group (n=24), the extracted teeth were fixed or transplanted immediately after extraction. At day 4, 1 and 2\u00a0weeks after transplantation, the teeth were examined by radiographic, histological, and immunohistochemical methods. The number of <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content> cells in the storage media was also counted.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Teeth immersed for 1\u00a0hour in milk showed the thinnest <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>. Immunohistochemistry of periostin and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>68 labeling suggested degradation of the extracellular matrix in the <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>. In the media used for immersion, more <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content> cells were observed in milk than in the other solutions. After transplantation, the <jats:styled-content style=\"fixed-case\">HBSS<\/jats:styled-content> and egg white groups maintained adequate thickness of <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content> but in the milk group, thinner <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content> and ankylosis were observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Adequate thickness of <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content> was maintained in the egg white group, whereas the milk group showed disturbance in the <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>, which may lead to ankylosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/edt.12351","type":"journal-article","created":{"date-parts":[[2017,5,25]],"date-time":"2017-05-25T16:49:46Z","timestamp":1495730986000},"page":"383-392","source":"Crossref","is-referenced-by-count":13,"title":["Effects of tooth storage media on periodontal 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