{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T10:46:54Z","timestamp":1760784414461},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,4,18]],"date-time":"2018-04-18T00:00:00Z","timestamp":1524009600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002657","name":"Istanbul \u00dcniversitesi","doi-asserted-by":"publisher","award":["58028"],"award-info":[{"award-number":["58028"]}],"id":[{"id":"10.13039\/501100002657","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Dental Traumatology"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background\/Aim<\/jats:title><jats:p>Ectoine is an amino acid that can preserve osmotic balance and has more preservative activity than other osmoregulators. There are no published reports on the osmoregulators\u2019 effects on viability or differentiation of dental stem cells. The aim of this study was to investigate the effect of Ectoine as a storage media on the viability and differentiation potential of human periodontal ligament mesenchymal stem cells (<jats:styled-content style=\"fixed-case\">hPDLMSC<\/jats:styled-content>s).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">hPDLMSC<\/jats:styled-content>s were obtained from impacted third molar teeth. The cells were isolated, submitted to trilineage differentiation, and characterized by flow cytometer (<jats:styled-content style=\"fixed-case\">FC<\/jats:styled-content>). <jats:styled-content style=\"fixed-case\">hPDLMSC<\/jats:styled-content>s were incubated with different media containing Ectoine, complete <jats:styled-content style=\"fixed-case\">DMEM<\/jats:styled-content> (c<jats:styled-content style=\"fixed-case\">DMEM<\/jats:styled-content>), Ectoine+c<jats:styled-content style=\"fixed-case\">DMEM<\/jats:styled-content>, milk, and tap water for 2, 6, 12, and 24\u00a0h. The cells were analyzed by <jats:styled-content style=\"fixed-case\">FC<\/jats:styled-content> for viability, early\u2010apoptosis, late apoptosis, and necrosis rates. Osteogenic and fibroblastic differentiation in <jats:styled-content style=\"fixed-case\">hPDLMSC<\/jats:styled-content>s were investigated by Alizarin red stain and vimentin expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All treated groups showed significant decreases in cell viability after 2\u00a0h. Significant increases were detected in the number of dead cells between 2 and 12\u00a0h in all groups except the Ectoine+c<jats:styled-content style=\"fixed-case\">DMEM<\/jats:styled-content> group. The deposition of mineral matrix nodules was significantly higher in cells cultured with Ectoine+c<jats:styled-content style=\"fixed-case\">DMEM<\/jats:styled-content> compared with the other media. Higher vimentin expressions were detected in cells cultured with c<jats:styled-content style=\"fixed-case\">DMEM<\/jats:styled-content> and Ectoine+c<jats:styled-content style=\"fixed-case\">DMEM<\/jats:styled-content> media, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Ectoine added to c<jats:styled-content style=\"fixed-case\">DMEM<\/jats:styled-content> media, promoted cell survival plus osteogenic and fibroblastic differentiation of <jats:styled-content style=\"fixed-case\">hPDLMSC<\/jats:styled-content>s.<\/jats:p><\/jats:sec>","DOI":"10.1111\/edt.12396","type":"journal-article","created":{"date-parts":[[2018,3,12]],"date-time":"2018-03-12T14:56:42Z","timestamp":1520866602000},"page":"188-200","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effect of c<scp>DMEM<\/scp> media containing Ectoine on human periodontal ligament mesenchymal stem cell survival and 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