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Sc<jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content> could be a promising agent for blocking osteoclast activity and preventing root resorption. The aim of this study was to evaluate the effect of Sc<jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content> on the viability of the <jats:styled-content style=\"fixed-case\">RAW<\/jats:styled-content> 264.7 macrophage lineage, osteoclast\u2010like maturation and the release of <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 and nitric oxide (<jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The viability of <jats:styled-content style=\"fixed-case\">RAW<\/jats:styled-content> 264.7 cells was determined by <jats:styled-content style=\"fixed-case\">MTT<\/jats:styled-content> and Alamar Blue assays after Sc<jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content> treatment for 24\u00a0hours. Sc<jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content> effects on <jats:styled-content style=\"fixed-case\">RANKL<\/jats:styled-content>\u2010induced osteoclast\u2010like maturation were assessed by tartrate\u2010resistant acid phosphatase (<jats:styled-content style=\"fixed-case\">TRAP<\/jats:styled-content>) staining and F\u2010actin ring formation. The supernatant was collected to detect the release of <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 using <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content> using a nitrite assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Sc<jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content> suppressed osteoclast\u2010like maturation by decreasing <jats:styled-content style=\"fixed-case\">TRAP<\/jats:styled-content> activity as well as F\u2010actin ring formation. Sc<jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content> at 10\u00a0\u03bcg\/mL showed the highest values of <jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content> release compared with all other groups (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). Lower levels of <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 were found for the negative control.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Sc<jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content> at 5\u00a0\u03bcg\/mL suppressed osteoclast\u2010like maturation in vitro and had no cytotoxic effect on <jats:styled-content style=\"fixed-case\">RAW<\/jats:styled-content> cell cultures.<\/jats:p><\/jats:sec>","DOI":"10.1111\/edt.12412","type":"journal-article","created":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T13:25:20Z","timestamp":1527859520000},"page":"329-335","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of Lectin (Sc<scp>LL<\/scp>) on osteoclast\u2010like multinucleated giant cells\u2019 maturation\u2014A preliminary in vitro study"],"prefix":"10.1111","volume":"34","author":[{"given":"Manuella V. P.","family":"Reis","sequence":"first","affiliation":[{"name":"Biomechanics Research Group Department of Operative Dentistry and Dental Materials Federal University of Uberl\u00e2ndia  Uberl\u00e2ndia Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela L.","family":"de Souza","sequence":"additional","affiliation":[{"name":"Biomechanics Research Group Department of Endodontics Federal University of Uberl\u00e2ndia  Uberl\u00e2ndia Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5653-8403","authenticated-orcid":false,"given":"Camilla C. 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