{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T19:36:00Z","timestamp":1788464160152,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Dental Traumatology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background\/Aim<\/jats:title><jats:p>The high rate of root resorption resulting from tooth replantation represents a serious clinical problem. In order to prevent ankylosis and replacement resorption, the contemporary literature highlights the importance of using a flexible stabilization for traumatized teeth. For this purpose, orthodontic devices may be promising for obtaining a better prognosis and periodontal repair. The aim of this study was to evaluate the effect of an active splinting protocol with controlled force in dog's teeth following replantation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Sixty premolar roots from three dogs were used. They were submitted to endodontic treatment, hemisected, atraumatically extracted and subsequently replanted. They were divided into four groups: Passive Stabilization (<jats:italic>n<\/jats:italic>\u00a0=\u00a020)\u2010after 20\u00a0min in a dry medium; Active Stabilization (<jats:italic>n<\/jats:italic>\u00a0=\u00a020)\u2010after 20\u00a0min in a dry medium; Negative control (<jats:italic>n<\/jats:italic>\u00a0=\u00a010)\u2010immediate replantation and passive Stabilization; and Positive control (<jats:italic>n<\/jats:italic>\u00a0=\u00a010)\u201090\u00a0min of extra\u2010alveolar time and passive Stabilization. The samples were collected and submitted to histologic processing. They were then evaluated for the count of inflammatory cells, expression of neurotrophin 4, osteoclasts, apoptotic cells and collagen fibres. The results were submitted to ANOVA or Kruskal\u2010Wallis statistical tests followed by Tukey or Dunn post\u2010tests (<jats:italic>\u03b1<\/jats:italic>\u00a0=\u00a05%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Passive Stabilization with orthodontic brackets without traction used after replantation had the highest number of inflammatory cells (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.0122), osteoclasts (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.0013) and percentage of collagen fibres in the periodontal ligament (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.0001) when compared to Active Stabilization with orthodontic brackets applying amild tensile force. Neurotrophin 4\u00a0had no statistically significant difference (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.05), regardless of the treatment. The apoptotic cells count revealed statistical differences (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.0001) between Active Stabilization (189.70\u00a0\u00b1\u00a047.99) and Positive Control (198.90\u00a0\u00b1\u00a088.92) when compared to Passive Stabilization (21.19\u00a0\u00b1\u00a032.94).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The active splinting protocol using orthodontic appliances generating a light and controlled force favoured periodontal ligament repair of replanted teeth.<\/jats:p><\/jats:sec>","DOI":"10.1111\/edt.12698","type":"journal-article","created":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T06:32:14Z","timestamp":1625207534000},"page":"758-771","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Periodontal ligament repair after active splinting of replanted dogs' teeth"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0230-1347","authenticated-orcid":false,"given":"Raquel Assed Bezerra","family":"Silva","sequence":"first","affiliation":[{"name":"Department of Pediatric Dentistry School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helo\u00edsa Aparecida Orsini","family":"Vieira","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6020-0392","authenticated-orcid":false,"given":"C\u00e9sar","family":"de Gregorio","sequence":"additional","affiliation":[{"name":"Department of Dentistry University Rey Juan Carlos Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0603-5437","authenticated-orcid":false,"given":"Nestor","family":"Cohenca","sequence":"additional","affiliation":[{"name":"Department of Endodontics University of Washington Seattle WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6866-0561","authenticated-orcid":false,"given":"Mar\u00edlia Pac\u00edfico","family":"Lucisano","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4876-6892","authenticated-orcid":false,"given":"Carolina Maschietto","family":"Pucinelli","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8559-532X","authenticated-orcid":false,"given":"Francisco Wanderley Garcia","family":"Paula\u2010Silva","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8802-6480","authenticated-orcid":false,"given":"Paulo","family":"Nelson\u2010Filho","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1419-3520","authenticated-orcid":false,"given":"F\u00e1bio Louren\u00e7o","family":"Romano","sequence":"additional","affiliation":[{"name":"Department 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