{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T05:14:37Z","timestamp":1737004477315,"version":"3.33.0"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T00:00:00Z","timestamp":1721001600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Dental Traumatology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Tooth avulsion necessitates swift replantation, for which the preservation of periodontal ligament (PDL) cell viability is paramount. Various storage media have been explored, yet a comparison between amniotic fluid (AF) obtained at different gestational stages (amniocentesis and full\u2010term) and HBSS is lacking.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>This study aims to evaluate AF (amniocentesis and full\u2010term) against HBSS in sustaining PDL cell viability and regulating apoptosis at different time points.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Periodontal fibroblasts cultured in \u03b1\u2010MEM were treated with 100% AF (amniocentesis), 100% AF (full\u2010term), and HBSS, incubated for 1, 3, 24, and 48\u2009h at 37\u00b0C, and assessed using the MTT assay for viability and AO\/EB staining for apoptosis, which was analyzed via fluorescent microscopy after 24\u2009h. Statistical analysis was conducted using one\u2010way ANOVA, multivariate ANOVA, and post hoc Tukey's multiple comparison tests (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Amniotic fluid (amniocentesis) exhibited the highest optical density (OD), which implies the highest cell viability across time intervals, followed by AF (full\u2010term) and HBSS. While HBSS maintained PDL morphology, both AF groups showed altered morphology. No cell death was observed after 24\u2009h.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Within the limitations of this study, both AF groups showed the potential to sustain PDL cell viability after 1, 3, 24, and 48\u2009h of storage. However, further investigation is warranted regarding their suitability as storage media.<\/jats:p><\/jats:sec>","DOI":"10.1111\/edt.12980","type":"journal-article","created":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T11:14:16Z","timestamp":1721042056000},"page":"82-92","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative in\u2010vitro analysis of amniotic Fluid's efficacy in sustaining viability and regulating apoptosis of periodontal fibroblasts versus <scp>HBSS<\/scp>"],"prefix":"10.1111","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3980-0163","authenticated-orcid":false,"given":"Gunica","family":"Harjai","sequence":"first","affiliation":[{"name":"Department of Paediatric and Preventive Dentistry JSS Dental College and Hospital, A Constituent College of JSS Academy of Higher Education &amp; Research  Mysore Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7115-8417","authenticated-orcid":false,"given":"Raghavendra","family":"Shanbhog","sequence":"additional","affiliation":[{"name":"Department of Paediatric and Preventive Dentistry JSS Dental College and Hospital, A Constituent College of JSS Academy of Higher Education &amp; Research  Mysore Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7,15]]},"reference":[{"volume-title":"Textbook and color atlas of traumatic injuries to the teeth","year":"2007","author":"Andreasen J","key":"e_1_2_11_2_1"},{"key":"e_1_2_11_3_1","first-page":"486","volume-title":"Textbook and color atlas of traumatic injuries to the teeth","author":"Andreasen JO","year":"2019"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/edt.12403"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/edt.12437"},{"volume-title":"Samhita Bijlani\u2014efficacy of ice apple, propolis and amniotic fluid in maintaining periodontal ligament cell viability of avulsed tooth: an in\u2010vitro evaluation","year":"2018","key":"e_1_2_11_6_1"},{"issue":"3","key":"e_1_2_11_7_1","first-page":"147","article-title":"Amniotic fluid stem cells and their application in cell\u2010based tissue regeneration","volume":"6","author":"Eslaminejad MB","year":"2012","journal-title":"Int J Fertil Steril"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-801238-3.64497-5"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-017-0582-6"},{"key":"e_1_2_11_10_1","unstructured":"Manual of Procedures The GAPPS Repository. 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