{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T09:27:13Z","timestamp":1751880433805},"reference-count":51,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,10,26]],"date-time":"2012-10-26T00:00:00Z","timestamp":1351209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Increased bone marrow angiogenesis is seen in several hematological malignancies, including acute myeloid leukemia (<jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content>). We used a co\u2010culture assay of endothelial and vascular smooth muscle cells (v<jats:styled-content style=\"fixed-case\">SMC<\/jats:styled-content>) to investigate the effects of <jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content>\u2010conditioned medium on capillary networks. We investigated primary <jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content> cells derived from 44 unselected patients and observed that for a large subset of patients, the constitutive cytokine release by the leukemic cells stimulated endothelial cell organization into capillary\u2010like networks, while there were only minor or no effects for other patients. We analyzed the constitutive <jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content> cell release of 31 cytokines for all the patients and performed a hierarchical cluster analysis of the cytokine profile which identified two major patient subsets that differed in their ability to enhance capillary\u2010like networks; increased capillary\u2010like networks was then associated with high constitutive release of several cytokines and especially high levels of several pro\u2010angiogenic chemokines. Significantly increased network formation was not seen for any of the 11 acute lymphoblastic leukemia patients investigated. The cytokine response by activated normal T cells inhibited endothelial network formation in our <jats:italic>in vitro<\/jats:italic> model of angiogenesis and activated normal monocytes had only a minor influence on tube formation. Our study shows that <jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content>\u2010derived cytokines can induce the organization of endothelial cells into vessel\u2010like structures.<\/jats:p>","DOI":"10.1111\/ejh.12016","type":"journal-article","created":{"date-parts":[[2012,10,9]],"date-time":"2012-10-09T13:31:51Z","timestamp":1349789511000},"page":"478-490","source":"Crossref","is-referenced-by-count":15,"title":["Soluble mediators released by acute myeloid leukemia cells increase capillary\u2010like networks"],"prefix":"10.1111","volume":"89","author":[{"given":"Kimberley J.","family":"Hatfield","sequence":"first","affiliation":[{"name":"Section for Hematology Department of Medicine Haukeland University Hospital Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lasse","family":"Evensen","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine University of Bergen Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e5kon","family":"Reikvam","sequence":"additional","affiliation":[{"name":"Department of Hematology Institute of Medicine, University of Bergen Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James B.","family":"Lorens","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine University of Bergen Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00d8ystein","family":"Bruserud","sequence":"additional","affiliation":[{"name":"Section for Hematology Department of Medicine Haukeland University Hospital Bergen Norway"},{"name":"Department of Hematology Institute of Medicine, University of Bergen Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,10,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.1.309"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.8.2637"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.10100995"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.019364"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/1042819032000159915"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428190410001663707"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-08-173104"},{"key":"e_1_2_7_9_1","first-page":"416","article-title":"Flt3\u2010mediated signaling in human acute myelogenous leukemia (AML) blasts: a functional characterization of Flt3\u2010ligand effects in AML cell populations with and without genetic Flt3 abnormalities","volume":"88","author":"Bruserud O","year":"2003","journal-title":"Haematologica"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0609.2003.00130.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404985"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568009054064651"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428190500054491"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404820"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.10900"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJHTS.S13459"},{"key":"e_1_2_7_17_1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1002\/cyto.a.20808","article-title":"A novel imaging\u2010based high\u2010throughput screening approach to anti\u2010angiogenic drug discovery","volume":"77","author":"Evensen L","year":"2010","journal-title":"Cytometry A"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005798"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-07-235358"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.23116"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2008.10.005"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2011.08960.x"},{"key":"e_1_2_7_23_1","first-page":"1783","article-title":"Proposals for the immunological classification of acute leukemias. European Group for the Immunological Characterization of Leukemias (EGIL)","volume":"9","author":"Bene MC","year":"1995","journal-title":"Leukemia"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(02)00227-8"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1089\/152581603322448231"},{"key":"e_1_2_7_26_1","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1684\/ecn.2009.0181","article-title":"Early pre\u2010engraftment, functional, in vitro responsiveness of T lymphocytes in allotransplanted, acute leukemia patients: proliferation and release of a broad profile of cytokines, possibly predictive of graft\u2010versus\u2010host disease","volume":"21","author":"Liseth K","year":"2010","journal-title":"Eur Cytokine Netw"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.2004.00307.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/82_2010_21"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/128608"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-01-315069"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2011.236"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2001.02722.x"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.10092"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2008.07411.x"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.22180"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.3.1455"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.10148"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.6.2240"},{"key":"e_1_2_7_39_1","first-page":"815","article-title":"Spectrum of tumor angiogenesis in the bone marrow of children with acute lymphoblastic leukemia","volume":"150","author":"Perez\u2010Atayde AR","year":"1997","journal-title":"Am J Pathol"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-03-1010"},{"key":"e_1_2_7_41_1","first-page":"4140","article-title":"Peripheral blood T lymphocytes and lymphocytes infiltrating human cancers express vascular endothelial growth factor: a potential role for T cells in angiogenesis","volume":"55","author":"Freeman MR","year":"1995","journal-title":"Cancer Res"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/3-7643-7311-3_9"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1586\/ehm.11.54"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-10-312934"},{"key":"e_1_2_7_45_1","first-page":"508","article-title":"Synergistic effects of vascular endothelial growth factor and basic fibroblast growth factor on the proliferation and cord formation of bovine capillary endothelial cells within collagen gels","volume":"69","author":"Goto F","year":"1993","journal-title":"Lab Invest"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1177\/172460080902400104"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402534"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13402-011-0017-9"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.2004.00333.x"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.2490"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-06-1731"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64058-8"}],"container-title":["European Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fejh.12016","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejh.12016","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T18:23:36Z","timestamp":1698776616000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ejh.12016"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,26]]},"references-count":51,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1111\/ejh.12016"],"URL":"https:\/\/doi.org\/10.1111\/ejh.12016","archive":["Portico"],"relation":{},"ISSN":["0902-4441","1600-0609"],"issn-type":[{"value":"0902-4441","type":"print"},{"value":"1600-0609","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,26]]}}}