{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T14:08:02Z","timestamp":1761919682641},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,5,24]],"date-time":"2013-05-24T00:00:00Z","timestamp":1369353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Deletions of the long arm of chromosome 6 are known to occur at relatively low frequency (3\u20136%) in chronic lymphocytic leukemia (<jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content>), and they are more frequently observed in 6q21. Few data have been reported regarding other bands on 6q involved by cytogenetic alterations in <jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content>. The cytogenetic study was performed in nuclei and metaphases obtained after stimulation with a combination of CpG\u2010oligonucleotide <jats:styled-content style=\"fixed-case\">DSP<\/jats:styled-content>30 and interleukin\u20102. Four bacterial artificial chromosome (<jats:styled-content style=\"fixed-case\">BAC<\/jats:styled-content>) clones mapping regions in bands 6q16, 6q23, 6q25, 6q27 were used as probes for fluorescence <jats:italic>in situ<\/jats:italic> hybridization in 107 <jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content> cases in order to analyze the occurrence and localization of 6q aberrations. We identified 11 cases (10.2%) with 6q deletion of 107 patients studied with <jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content>. The trends of survival curves and the treatment\u2010free intervals (<jats:styled-content style=\"fixed-case\">TFI<\/jats:styled-content>) of patients with deletion suggest a better outcome than the other cytogenetic risk groups. We observed two subgroups with 6q deletion as the sole anomaly: two cases with 6q16 deletion, and three cases with 6q25.2\u201327 deletion. There were differences of age, stage, and <jats:styled-content style=\"fixed-case\">TFI<\/jats:styled-content> between both subgroups. By using <jats:styled-content style=\"fixed-case\">BAC<\/jats:styled-content> probes, we observed that 6q deletion has a higher frequency in <jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content> and is linked with a good prognosis. In addition, it was observed that the deletion in 6q16 appears to be the most frequent and, if present as the only abnormality, it could be associated with a most widespread disease.<\/jats:p>","DOI":"10.1111\/ejh.12115","type":"journal-article","created":{"date-parts":[[2013,4,6]],"date-time":"2013-04-06T06:43:59Z","timestamp":1365230639000},"page":"10-19","source":"Crossref","is-referenced-by-count":8,"title":["6q deletion detected by fluorescence <i>in situ<\/i> hybridization using bacterial artificial chromosome in chronic lymphocytic leukemia"],"prefix":"10.1111","volume":"91","author":[{"given":"Alessia","family":"Dalsass","sequence":"first","affiliation":[{"name":"SC Ematologia Ospedale C. G. Mazzoni Ascoli Piceno Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Mestichelli","sequence":"additional","affiliation":[{"name":"SC Ematologia Ospedale C. G. 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