{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T12:05:07Z","timestamp":1788264307302,"version":"build-2803163510"},"reference-count":19,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,6,28]],"date-time":"2013-06-28T00:00:00Z","timestamp":1372377600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2013,8]]},"abstract":"Abstract<\/jats:title>\n \n Systemic mastocytosis (\n SM<\/jats:styled-content>\n ) is characterized by the growth of neoplastic mast cells (\n MC<\/jats:styled-content>\n s). Most adults with indolent\n SM<\/jats:styled-content>\n carry the\n \n KIT<\/jats:styled-content>\n <\/jats:italic>\n D816V mutation. We recently introduced the D816V+ allele fraction as a disease marker in\n SM<\/jats:styled-content>\n using a sensitive and quantitative\n \n KIT<\/jats:styled-content>\n <\/jats:italic>\n D816V mutation analysis that consistently allows mutation detection in peripheral blood (\n PB<\/jats:styled-content>\n ) and bone marrow (\n BM<\/jats:styled-content>\n ). The D816V+ allele fraction represents a quantitative measure which allows\n \n KIT<\/jats:styled-content>\n <\/jats:italic>\n D816V\u2010positivity to be analyzed as a continuous variable instead of a categorical variable (negative\/positive) as previously described. Serum tryptase represents an established disease marker in\n SM<\/jats:styled-content>\n , and it remains to be tested whether tryptase and the D816V+ allele fraction are associated or represent independent disease markers. In this study, correlation analysis between serum tryptase, the D816V+ allele fraction in\n PB<\/jats:styled-content>\n and\n BM<\/jats:styled-content>\n , and the\n MC<\/jats:styled-content>\n fraction was performed in 57 indolent systemic mastocytosis (\n ISM<\/jats:styled-content>\n ) patients. We detected significant correlations between the D816V+ allele fraction, the mature neoplastic\n MC<\/jats:styled-content>\n fraction, and serum tryptase which represent three different biological measures of disease burden. Mutation analysis was performed in two or more\n PB<\/jats:styled-content>\n samples in 39 patients, and the results demonstrated high stability with no overall tendency to increasing D816V+ allele fractions over time. Considerable variation was nevertheless observed in the correlation analyses. Serum tryptase reflects the mature\n MC<\/jats:styled-content>\n load, whereas the D816V+ allele fraction includes cells other than mature\n MC<\/jats:styled-content>\n s to a variable extent. We conclude that tryptase and the D816V+ allele fraction represent different, although correlated, measures of disease status in\n SM<\/jats:styled-content>\n .\n <\/jats:p>","DOI":"10.1111\/ejh.12128","type":"journal-article","created":{"date-parts":[[2013,4,27]],"date-time":"2013-04-27T10:00:48Z","timestamp":1367056848000},"page":"106-111","source":"Crossref","is-referenced-by-count":26,"title":["Serum tryptase correlates with the\n \n KIT<\/scp>\n <\/i>\n D816V mutation burden in adults with indolent systemic mastocytosis"],"prefix":"10.1111","volume":"91","author":[{"given":"Thomas","family":"Kristensen","sequence":"first","affiliation":[{"name":"Department of Pathology Odense University Hospital Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sigurd","family":"Broesby\u2010Olsen","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy Centre Odense University Hospital Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanne","family":"Vestergaard","sequence":"additional","affiliation":[{"name":"Department of Haematology Odense University Hospital Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carsten","family":"Bindslev\u2010Jensen","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy Centre Odense University Hospital Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael Boe","family":"M\u00f8ller","sequence":"additional","affiliation":[{"name":"Department of Pathology Odense University Hospital Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"the Mastocytosis Centre Odense University Hospital (MastOUH)","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,6,28]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"54","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Horny HP","year":"2008"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02550.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.2007.01807.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.iac.2006.05.010"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.2009.02184.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-04-015545"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-02-205237"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.23.10560"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2010.10.004"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.2012.01789.x"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.10072"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0320(20011101)45:3<194::AID-CYTO1163>3.0.CO;2-C"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000059404"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-01-0203"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-472X(99)00145-9"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.05.003"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2011.293"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2010.02477.x"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejh.12043"}],"container-title":["European Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fejh.12128","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejh.12128","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T19:33:54Z","timestamp":1696275234000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ejh.12128"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6,28]]},"references-count":19,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["10.1111\/ejh.12128"],"URL":"https:\/\/doi.org\/10.1111\/ejh.12128","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.718009429.793476475","asserted-by":"object"}]},"ISSN":["0902-4441","1600-0609"],"issn-type":[{"value":"0902-4441","type":"print"},{"value":"1600-0609","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6,28]]}}}