{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:04:18Z","timestamp":1791288258961,"version":"4.3.0"},"reference-count":19,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,6,15]],"date-time":"2013-06-15T00:00:00Z","timestamp":1371254400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Regulatory <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>reg) cells are essential for maintaining immune tolerance. High <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>reg frequencies have been reported in peripheral blood and tissue samples of patients with solid tumors while their role in lymphomas, including diffuse large <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\u2010cell lymphoma (<jats:styled-content style=\"fixed-case\">DLBCL<\/jats:styled-content>) has not been clearly established. In this study, we analyzed the circulating <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>reg numbers in 27 patients with newly diagnosed <jats:styled-content style=\"fixed-case\">DLBCL<\/jats:styled-content> and 17 healthy individuals. Tregs were detected by flow cytometry based on <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup><jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>25<jats:sup>high<\/jats:sup><jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>ox<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>3<jats:sup>+<\/jats:sup> phenotype. In addition, the expression of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45<jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> HLA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DR<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> CD<\/jats:styled-content>62L, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>39, and <jats:styled-content style=\"fixed-case\">CTLA<\/jats:styled-content>4 was analyzed. The number of circulating <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>reg cells was lower in patients with <jats:styled-content style=\"fixed-case\">DLBCL<\/jats:styled-content> than in healthy controls: median 23 (range, 4\u2013107)\/\u03bcL vs. 41 (19\u2013104)\/\u03bcL (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.04). In particular, the number of <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>regs expressing <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45<jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content> (na\u00efve Tregs), <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DR<\/jats:styled-content> (marker of activation), and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>62L (<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>\u2010selectin) was decreased in the <jats:styled-content style=\"fixed-case\">DLBCL<\/jats:styled-content> group. Lower (below median) number of circulating Tregs was associated with reduced chance of achieving complete remission (29% vs. 69%, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.05) and reduced probability of even\u2010free survival (24% vs. 84% at 1\u00a0yr, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.0004), independently on the International Prognostic Index. We conclude that low number of circulating Tregs may be associated with poor prognosis in patients with <jats:styled-content style=\"fixed-case\">DLBCL<\/jats:styled-content>. However, our observations require confirmation in larger patient population.<\/jats:p>","DOI":"10.1111\/ejh.12144","type":"journal-article","created":{"date-parts":[[2013,5,17]],"date-time":"2013-05-17T07:23:48Z","timestamp":1368775428000},"page":"122-128","source":"Crossref","is-referenced-by-count":32,"title":["Association of circulating regulatory <scp>T<\/scp> cell number with the incidence and prognosis of diffuse large <scp>B<\/scp>\u2010cell lymphoma"],"prefix":"10.1111","volume":"91","author":[{"given":"Magdalena","family":"G\u0142owala\u2010Kosi\u0144ska","sequence":"first","affiliation":[{"name":"Department of Bone Marrow Transplantation Maria Sklodowska\u2010Curie Memorial Cancer Center and Institute of Oncology Gliwice Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agata","family":"Chwieduk","sequence":"additional","affiliation":[{"name":"Department of Bone Marrow Transplantation Maria Sklodowska\u2010Curie Memorial Cancer Center and Institute of Oncology Gliwice Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaros\u0142aw","family":"Nieckula","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Oncology Maria Sklodowska\u2010Curie Memorial Cancer Center and Institute of Oncology Gliwice Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Sadu\u015b\u2010Wojciechowska","sequence":"additional","affiliation":[{"name":"Department of Bone Marrow Transplantation Maria Sklodowska\u2010Curie Memorial Cancer Center and Institute of Oncology Gliwice Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Grosicki","sequence":"additional","affiliation":[{"name":"Department of Haematology Municipal Hospital Chorzow Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra","family":"Rusin","sequence":"additional","affiliation":[{"name":"Center for Translational Research and Molecular Biology of Cancer Maria Sklodowska\u2010Curie Memorial Cancer Center and Institute of Oncology Gliwice Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"El\u017cbieta","family":"Nowara","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Oncology Maria Sklodowska\u2010Curie Memorial Cancer Center and Institute of Oncology Gliwice Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Giebel","sequence":"additional","affiliation":[{"name":"Department of Bone Marrow Transplantation Maria Sklodowska\u2010Curie Memorial Cancer Center and Institute of Oncology Gliwice 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