{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T14:28:28Z","timestamp":1780669708785,"version":"3.54.1"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,2,10]],"date-time":"2014-02-10T00:00:00Z","timestamp":1391990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Folate deficiency is assessed by serum and red blood cell folate measurements. Nevertheless, no consensus for the lower limit of serum folate reference values exists. We investigated the appropriate use of <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> folate to detect folate deficiency and the relationship between serum and <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> folate and with other parameters such as vitamin <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content><jats:sub>12<\/jats:sub> and homocysteine in order to propose serum folate cut\u2010off values.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Retrospectively, 63\u00a0113 and 20\u00a0459 results of serum and <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> folate were collected. If present, the results of red cell indices, vitamin <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content><jats:sub>12<\/jats:sub> and homocysteine were also collected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A significantly positive correlation between serum and <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> folate was demonstrated. A significant effect of serum folate levels under 6\u00a0\u03bcg\/<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content> (or 14\u00a0n<jats:sc>m<\/jats:sc>) was observed on <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> indices. A relation was found between vitamin <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content><jats:sub>12<\/jats:sub> and folate, for serum and <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>. A significant rise in homocysteine concentrations was observed for serum folate levels under 8\u00a0\u03bcg\/<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content> (or 18\u00a0n<jats:sc>m<\/jats:sc>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>To observe haematological abnormalities, folate deficiency should be profound. Serum folate levels under 8\u00a0\u03bcg\/<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content> (or 18\u00a0n<jats:sc>m<\/jats:sc>) should be considered as a decision limit for folate depletion because a positive effect on homocysteine was observed. Fasting serum folate concentration should be preferred for assessing folate status. Our results suggest that the need for <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> folate testing is less meaningful.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ejh.12237","type":"journal-article","created":{"date-parts":[[2013,12,18]],"date-time":"2013-12-18T15:31:17Z","timestamp":1387380677000},"page":"354-359","source":"Crossref","is-referenced-by-count":31,"title":["Serum and red blood cell folate testing for folate deficiency: new features?"],"prefix":"10.1111","volume":"92","author":[{"given":"Edward","family":"De Bruyn","sequence":"first","affiliation":[{"name":"Department of Clinical Chemistry, H\u00f4pital Erasme Universit\u00e9 Libre de Bruxelles Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9atrice","family":"Gulbis","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, H\u00f4pital Erasme Universit\u00e9 Libre de Bruxelles Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Cotton","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, H\u00f4pital Erasme Universit\u00e9 Libre de Bruxelles Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,2,10]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"1143","volume-title":"Wintrobe's Clinical Hematology","author":"Carmel R","year":"2009"},{"key":"e_1_2_6_3_1","first-page":"492","volume-title":"Tietz, Fundamentals of Clinical Chemistry","author":"Shenkin A","year":"2008"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/mgme.2000.3027"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.19.1.17"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/129.4.913"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN20051618"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"14653","DOI":"10.1016\/S0021-9258(17)38620-9","article-title":"The binding of folyl\u2010 and antifolylpolyglutamates to hemoglobin","volume":"260","author":"Benesch RE","year":"1985","journal-title":"J Biol Chem"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"1978","DOI":"10.1093\/clinchem\/46.12.1978","article-title":"Erythrocyte folate analysis: saponin added during lysis of whole blood can increase apparent folate concentrations, depending on hemolysate pH","volume":"46","author":"Anthony AJA","year":"2000","journal-title":"Clin Chem"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1309\/L2U6HH5KAYG48L40"},{"key":"e_1_2_6_11_1","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1093\/clinchem\/23.8.1505","article-title":"Inadequate sample preparation technique as a source of error in determination of erythrocyte folate by competitive binding radioassay","volume":"23","author":"Netteland B","year":"1977","journal-title":"Clin Chem"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/44.9.1886"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1993.03510220049033"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.041210"},{"key":"e_1_2_6_15_1","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1093\/clinchem\/44.7.1545","article-title":"Plasma folic acid cutoff value, derived from its relationship with homocyst(e)ine","volume":"44","author":"Brouwer DAJ","year":"1998","journal-title":"Clin Chem"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/136.1.153"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.2143\/TVG.59.23.5001750"},{"key":"e_1_2_6_18_1","volume-title":"Serum and Red Blood Cell Folate Concentrations for Assessing Folate Status in Populations. 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