{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T20:07:50Z","timestamp":1788379670989,"version":"build-2803163510"},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,1,10]],"date-time":"2014-01-10T00:00:00Z","timestamp":1389312000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2014,3]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>The \u03b1(0)\u2010thalassemia South\u2010East Asian (SEA<\/jats:styled-content>)\u2010type deletion is the most common genetic disorder in the Asian population. Couples who are both carriers have a 25% chance of conceiving Bart's hydrops fetalis. Therefore, results from carrier screening and prenatal diagnosis frequently need to be available rapidly. The aim of this study was to implement a droplet digital polymerase chain reaction (ddPCR<\/jats:styled-content>) for diagnosis of \u03b1(0)\u2010thalassemia SEA<\/jats:styled-content>\u2010type deletion.<\/jats:p><\/jats:sec>Methods<\/jats:title>The wild\u2010type \u03b1\u2010globin gene allele and \u03b1(0)\u2010thalassemia SEA<\/jats:styled-content> allele were quantified in DNA<\/jats:styled-content> samples of 20 normal individuals, 15 samples with \u03b1(0)\u2010thalassemia SEA<\/jats:styled-content> trait, and 8 samples with Bart's hydrops fetalis using the ddPCR<\/jats:styled-content>. The DNA<\/jats:styled-content> copy number of wild\u2010type \u03b1\u2010globin gene allele and \u03b1(0)\u2010thalassemia SEA<\/jats:styled-content> allele was then calculated using the Quantasoft analysis software.<\/jats:p><\/jats:sec>Results<\/jats:title>The mean \u00b1 standard deviation (SD<\/jats:styled-content>) ratio of wild\u2010type \u03b1\u2010globin gene allele and \u03b1(0)\u2010thalassemia SEA<\/jats:styled-content> allele among normal individuals, samples with \u03b1(0)\u2010thalassemia SEA<\/jats:styled-content> trait, and Bart's hydrops fetalis were clearly distinguished with levels of 1.78\u00a0\u00b1\u00a00.49, 0.85\u00a0\u00b1\u00a00.14, and 0.03\u00a0\u00b1\u00a00.03, respectively.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>The ddPCR<\/jats:styled-content> may be one alternative technology available for routine clinical diagnosis of \u03b1(0)\u2010thalassemia SEA<\/jats:styled-content>\u2010type deletion and prenatal diagnosis of Bart's hydrops fetalis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ejh.12246","type":"journal-article","created":{"date-parts":[[2013,12,14]],"date-time":"2013-12-14T15:18:57Z","timestamp":1387034337000},"page":"244-248","source":"Crossref","is-referenced-by-count":18,"title":["Detection of alpha(0)\u2010thalassemia South\u2010East Asian\u2010type deletion by droplet digital PCR<\/scp>"],"prefix":"10.1111","volume":"92","author":[{"given":"Sakorn","family":"Pornprasert","sequence":"first","affiliation":[{"name":"Department of Medical Technology Faculty of Associated Medical Sciences Chiang Mai University Chiang Mai Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Watcharee","family":"Prasing","sequence":"additional","affiliation":[{"name":"Department of Medical Technology Faculty of Associated Medical Sciences Chiang Mai University Chiang Mai 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