{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T06:12:07Z","timestamp":1696313527947},"reference-count":10,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,5,17]],"date-time":"2014-05-17T00:00:00Z","timestamp":1400284800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2015,2]]},"abstract":"Abstract<\/jats:title>Juvenile myelomonocytic leukemia (JMML<\/jats:styled-content>) is a clonal disease arising from abnormal hematopoietic stem cells, although the involvement of lymphoid lineage differs among reported cases. Here, we present a case of JMML<\/jats:styled-content> with a KRAS<\/jats:styled-content><\/jats:italic> G13D mutation. The mutation was detected in various hematopoietic lineages, including T and B lymphocytes and also in lineage\u2212<\/jats:sup> CD<\/jats:styled-content>34+<\/jats:sup>CD<\/jats:styled-content>38\u2212<\/jats:sup> hematopoietic stem cells, showing a different percentage of affected cells in each lineage. Single cell\u2010based analysis of hematopoietic cells revealed the loss of wild\u2010type KRAS<\/jats:styled-content><\/jats:italic> in a significant proportion of G13D\u2010harboring cells. The percentage of loss of heterozygosity (LOH<\/jats:styled-content>)\/non\u2010LOH<\/jats:styled-content> cells showed lineage\u2010dependent skewing in hematopoietic cells. The loss of the wild\u2010type KRAS<\/jats:italic> allele may be a common secondary genetic change in KRAS<\/jats:styled-content><\/jats:italic>\u2010related JMML<\/jats:styled-content> and may affect the differentiation behavior of early JMML<\/jats:styled-content> progenitors.<\/jats:p>","DOI":"10.1111\/ejh.12355","type":"journal-article","created":{"date-parts":[[2014,4,26]],"date-time":"2014-04-26T15:33:57Z","timestamp":1398526437000},"page":"177-181","source":"Crossref","is-referenced-by-count":0,"title":["Lineage\u2010dependent skewing of loss of heterozygosity (LOH<\/scp>) of KRAS<\/scp><\/i> gene in a case of juvenile myelomonocytic leukemia"],"prefix":"10.1111","volume":"94","author":[{"given":"Kiriko","family":"Tokuda","sequence":"first","affiliation":[{"name":"Department of Pediatrics Ehime University Graduate School of Medicine Ehime Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minenori","family":"Eguchi\u2010Ishimae","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Ehime University Graduate School of Medicine Ehime Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidehiko","family":"Iwabuki","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Ehime University Graduate School of Medicine Ehime Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanae","family":"Kawakami","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Ehime University Graduate School of Medicine Ehime Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisamichi","family":"Tauchi","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Ehime University Graduate School of Medicine Ehime Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eiichi","family":"Ishii","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Ehime University Graduate School of Medicine Ehime Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariko","family":"Eguchi","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Ehime University Graduate School of Medicine Ehime Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,5,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2010.1.357"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2010.08525.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2008.07104.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401240"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2006.050104"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(200004)30:4<1060::AID-IMMU1060>3.0.CO;2-2"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(02)00178-9"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.34439"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.24797"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.11503"}],"container-title":["European Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fejh.12355","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejh.12355","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T05:50:55Z","timestamp":1696225855000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ejh.12355"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5,17]]},"references-count":10,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,2]]}},"alternative-id":["10.1111\/ejh.12355"],"URL":"https:\/\/doi.org\/10.1111\/ejh.12355","archive":["Portico"],"relation":{},"ISSN":["0902-4441","1600-0609"],"issn-type":[{"value":"0902-4441","type":"print"},{"value":"1600-0609","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,5,17]]}}}