{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T02:28:24Z","timestamp":1791167304760,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,10,31]],"date-time":"2014-10-31T00:00:00Z","timestamp":1414713600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"the Internal Grant Agency of the Ministry of Health of the Czech Republic","award":["NT\/13836-4\/2012"],"award-info":[{"award-number":["NT\/13836-4\/2012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Downregulation of cereblon (<jats:italic>CRBN<\/jats:italic>) gene expression is associated with resistance to the immunomodulatory drug lenalidomide and poor survival outcomes in multiple myeloma (MM) patients. However, the importance of <jats:italic>CRBN<\/jats:italic> gene expression in patients with myelodysplastic syndrome (MDS) and its impact on lenalidomide therapy are not clear. In this study, we evaluate cereblon expression in mononuclear cells isolated from bone marrow [23 lower risk MDS patients with isolated 5q deletion (5q\u2010), 37 lower risk MDS patients with chromosome 5 without the deletion of long arms (non\u20105q\u2010), and 24 healthy controls] and from peripheral blood (38 patients with 5q\u2010, 52 non\u20105q\u2010 patients and 25 healthy controls) to gain insight into, firstly, the role of cereblon in lower risk MDS patients with or without 5q deletion and, secondly, into the mechanisms of lenalidomide action. Patients with 5q\u2010 lower risk MDS have the highest levels of CRBN <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> in comparison with both lower risk MDS without the deletion of long arms of chromosome 5 and healthy controls. <jats:italic>CRBN<\/jats:italic> gene expression was measured using the quantitative TaqMan real\u2010time PCR. High levels of CRBN <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> were detected in all lenalidomide responders during the course of therapy. A significant decrease of the CRBN <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> level during lenalidomide treatment is associated with loss of response to treatment and disease progression. These results suggest that, similar to the treatment of MM, high levels of full\u2010length CRBN <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> in lower risk 5q\u2010 patients are necessary for the efficacy of lenalidomide.<\/jats:p>","DOI":"10.1111\/ejh.12457","type":"journal-article","created":{"date-parts":[[2014,10,4]],"date-time":"2014-10-04T13:43:27Z","timestamp":1412430207000},"page":"27-34","source":"Crossref","is-referenced-by-count":26,"title":["High level of full\u2010length cereblon mRNA in lower risk myelodysplastic syndrome with isolated 5q deletion is implicated in the efficacy of lenalidomide"],"prefix":"10.1111","volume":"95","author":[{"given":"Anna","family":"Jonasova","sequence":"first","affiliation":[{"name":"1st Medical Clinic General University Hospital Prague Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radka","family":"Bokorova","sequence":"additional","affiliation":[{"name":"Institute of 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