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We investigated its effect on regulation of micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>) expression profiles in del(5q) patients with <jats:styled-content style=\"fixed-case\">MDS <\/jats:styled-content><jats:italic>in vivo<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression microarrays to study changes in mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> levels in peripheral blood <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>14+ monocytes collected from patients before and during lenalidomide treatment and compared them with those from healthy donors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Before treatment, we observed strong upregulation of pro\u2010apoptotic miR\u201034a and miR\u201034a* that diminished during lenalidomide exposure. Upregulation of <jats:styled-content style=\"fixed-case\">HOX<\/jats:styled-content>\u2010related miR\u2010196b and erythroid\u2010specific miR\u2010451 seen in untreated patients remained unchanged after the treatment. At the time of hematologic response, expression of several mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s clustering to the 14q32 locus was reduced. Additionally, we focused more deeply on mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s from the 5q commonly deleted region and found that levels of miR\u2010378 and miR\u2010378* followed haploinsufficiency trend.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This report describes changes in mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression in del(5q) patients with <jats:styled-content style=\"fixed-case\">MDS<\/jats:styled-content> treated with lenalidomide, likely arising from deregulation of pathways implicated in lenalidomide action.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ejh.12458","type":"journal-article","created":{"date-parts":[[2014,10,7]],"date-time":"2014-10-07T09:01:33Z","timestamp":1412672493000},"page":"35-43","source":"Crossref","is-referenced-by-count":18,"title":["Genome\u2010wide mi<scp>RNA<\/scp> profiling in myelodysplastic syndrome with del(5q) treated with 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