{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,31]],"date-time":"2025-08-31T10:03:19Z","timestamp":1756634599485},"reference-count":12,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,7,26]],"date-time":"2015-07-26T00:00:00Z","timestamp":1437868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The aim of our study was to analyse whether the \u03ba\/\u03bb free light chain ratio reference range for screening for Bence Jones proteinuria should be dependent on the estimated glomerular filtration rate (<jats:styled-content style=\"fixed-case\">eGFR<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The serum \u03ba\/\u03bb free light chain ratio, <jats:styled-content style=\"fixed-case\">eGFR<\/jats:styled-content>, serum M\u2010protein and Bence Jones protein were measured in 544 patients for whom Bence Jones protein analysis was ordered.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the population of patients without Bence Jones proteinuria or a M\u2010protein (<jats:italic>n<\/jats:italic>\u00a0=\u00a0402), there is no gradual increase in \u03ba\/\u03bb free light chain ratio with diminishing <jats:styled-content style=\"fixed-case\">eGFR<\/jats:styled-content>. The \u03ba\/\u03bb free light chain ratio in this group was 0.56\u20131.86 (95% interval). With this diagnostic reference range of the \u03ba\/\u03bb ratio, 105 of the 110 patients with Bence Jones protein could be identified correctly. Only five patients with Bence Jones proteinuria (&lt;0.17\u00a0g\/L) were missed, without diagnostic or therapeutic consequences. In 36 patients (6.6%), an abnormal \u03ba\/\u03bb free light chain ratio was measured without the presence of Bence Jones proteinuria.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>A \u03ba\/\u03bb free light chain ratio in serum can be used safely and efficiently to select urine samples which should be analysed for Bence Jones proteinuria with an electrophoresis\/immunofixation technique. Using this diagnostic reference range, the number of urine samples which should be analysed by electrophoresis\/immunofixation could be reduced by 74%. The diagnostic reference interval can be determined best in a group of patients for whom Bence Jones analysis is indicated. For calculation of this reference range, the <jats:styled-content style=\"fixed-case\">eGFR<\/jats:styled-content> value does not need to be taken into account.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ejh.12632","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T15:27:34Z","timestamp":1436887654000},"page":"527-531","source":"Crossref","is-referenced-by-count":3,"title":["Diagnostic reference range of \u03ba\/\u03bb free light chain ratio to screen for Bence Jones proteinuria is not significantly influenced by <scp>GFR<\/scp>"],"prefix":"10.1111","volume":"96","author":[{"given":"Yvonne","family":"Schmidt\u2010Hieltjes","sequence":"first","affiliation":[{"name":"Department of Clinical Chemistry and Haematology Rijnstate Hospital Arnhem the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clemens","family":"Elshof","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Haematology Rijnstate Hospital Arnhem the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lian","family":"Roovers","sequence":"additional","affiliation":[{"name":"Department of Clinical Research Rijnstate Hospital Arnhem the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janneke","family":"Ruinemans\u2010Koerts","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Haematology Rijnstate Hospital Arnhem the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,7,26]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2009.126664"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1258\/000456307782268084"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(14)70442-5"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404284"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/48.9.1437"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.02290508"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPR2M4EUYNHLGM"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2006.077180"},{"key":"e_1_2_4_10_1","volume-title":"Defining, Establishing, and Verifying Reference Intervals in the Clinical Laboratory; Proposed Guideline. 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