{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T11:21:34Z","timestamp":1722856894680},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,6,9]],"date-time":"2016-06-09T00:00:00Z","timestamp":1465430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001823","name":"Ministerstvo \u0160kolstv\u00ed, Ml\u00e1de\u017ee a T\u011blov\u00fdchovy","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Aldehyde dehydrogenase (<jats:styled-content style=\"fixed-case\">ALDH<\/jats:styled-content>) is highly active in physiological stem cells as well as in tumor\u2010initiating cells of some malignancies including multiple myeloma (<jats:styled-content style=\"fixed-case\">MM<\/jats:styled-content>). Finding higher activity of <jats:styled-content style=\"fixed-case\">ALDH<\/jats:styled-content> in some cell subsets in monoclonal gammopathies (<jats:styled-content style=\"fixed-case\">MG<\/jats:styled-content>) could identify potential source of myeloma\u2010initiating cells (<jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>s).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Bone marrow of 12 <jats:styled-content style=\"fixed-case\">MM<\/jats:styled-content>, 9 monoclonal gammopathy of undetermined significance (<jats:styled-content style=\"fixed-case\">MGUS<\/jats:styled-content>), and 10 healthy donors (<jats:styled-content style=\"fixed-case\">HD<\/jats:styled-content>) were analyzed by flow cytometry. <jats:styled-content style=\"fixed-case\">ALDH<\/jats:styled-content> activity of B\u2010cells and plasma cells (<jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>) was analyzed using Aldefluor.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Similar changes of <jats:styled-content style=\"fixed-case\">ALDH<\/jats:styled-content> activity were found during B\u2010cell development in <jats:styled-content style=\"fixed-case\">HD<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MG<\/jats:styled-content>. Decreasing of <jats:styled-content style=\"fixed-case\">ALDH<\/jats:styled-content> activity from immature to na\u00efve B\u2010cells was found. In postgerminal stages, the activity started to increase, and in <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>s, the <jats:styled-content style=\"fixed-case\">ALDH<\/jats:styled-content> activity was the same as in immature B\u2010cells. Increased <jats:styled-content style=\"fixed-case\">ALDH<\/jats:styled-content> activity of all <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content> subsets compared to na\u00efve B\u2010cells was found in <jats:styled-content style=\"fixed-case\">MM<\/jats:styled-content> as well as in <jats:styled-content style=\"fixed-case\">HD<\/jats:styled-content>, while in <jats:styled-content style=\"fixed-case\">MGUS<\/jats:styled-content>, only <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>19<jats:sup>\u2212<\/jats:sup><jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>s have higher <jats:styled-content style=\"fixed-case\">ALDH<\/jats:styled-content> activity. In <jats:styled-content style=\"fixed-case\">HD<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> ALDH<\/jats:styled-content> activity was higher in <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>19<jats:sup>+<\/jats:sup><jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>s compared with <jats:styled-content style=\"fixed-case\">MG<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results indicate that changes of <jats:styled-content style=\"fixed-case\">ALDH<\/jats:styled-content> activity are the natural phenomenon in B\u2010cell development; thus, high <jats:styled-content style=\"fixed-case\">ALDH<\/jats:styled-content> activity as a single marker is not appropriate for <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>s 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