{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T17:56:24Z","timestamp":1755798984754,"version":"3.37.3"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2017,5]]},"abstract":"Abstract<\/jats:title>Objectives<\/jats:title>We previously reported loss of heterozygosity on 1p in chronic myelogenous leukemia (CML<\/jats:styled-content>). We analyzed promoter methylation and mutation of tumor suppressor genes on 1p36 in CML<\/jats:styled-content>.<\/jats:p><\/jats:sec>Methods<\/jats:title>We performed methylation\u2010specific PCR<\/jats:styled-content> (MS<\/jats:styled-content>\u2010PCR<\/jats:styled-content>) analysis of the PRDM<\/jats:styled-content>2, RUNX<\/jats:styled-content>3,<\/jats:italic> and TP<\/jats:styled-content>73<\/jats:italic> genes in 61 patients with CML<\/jats:styled-content> (43 chronic phase, CP<\/jats:styled-content>; two accelerated phase; and 16 blast crisis, BC<\/jats:styled-content>). Oxidative MS<\/jats:styled-content>\u2010PCR<\/jats:styled-content>, PCR<\/jats:styled-content>\u2010single\u2010strand conformation polymorphism, and real\u2010time reverse transcriptase PCR<\/jats:styled-content> were also analyzed. K\u2010562 cells were grown in the presence of 5\u2010Aza\u2010dC<\/jats:styled-content> and trichostatin A.<\/jats:p><\/jats:sec>Results<\/jats:title>Methylation of the PRDM<\/jats:styled-content>2, RUNX<\/jats:styled-content>3,<\/jats:italic> and TP<\/jats:styled-content>73<\/jats:italic> genes was detected in 24\/60 (40%), 21\/61 (34%), and 28\/60 (47%) patients, respectively. Methylation of all three genes was detected in 19\/59 (32%) patients. Methylation was more frequent in BC<\/jats:styled-content> than in CP<\/jats:styled-content>. Oxidative MS<\/jats:styled-content>\u2010PCR<\/jats:styled-content> analysis detected 5\u2010mC<\/jats:styled-content> in the PRDM<\/jats:styled-content>2, RUNX<\/jats:styled-content>3,<\/jats:italic> and TP<\/jats:styled-content>73<\/jats:italic> genes in 10\/22 (45%), 15\/21 (71%), and 16\/26 (62%) samples with methylation detected by MS<\/jats:styled-content>\u2010PCR<\/jats:styled-content>, respectively. Decreased expression was observed in several samples with methylation, while no mutations were found in the genes. Treatment of K\u2010562 cells induced growth suppression, demethylation, and reexpression of the PRDM<\/jats:styled-content>2<\/jats:italic> and RUNX<\/jats:styled-content>3<\/jats:italic> genes.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Multiple tumor suppressor genes on 1p were inactivated in CML<\/jats:styled-content> by methylation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ejh.12857","type":"journal-article","created":{"date-parts":[[2017,1,27]],"date-time":"2017-01-27T21:38:05Z","timestamp":1485553085000},"page":"467-477","source":"Crossref","is-referenced-by-count":3,"title":["Tumor suppressor gene methylation on the short arm of chromosome 1 in chronic myelogenous leukemia"],"prefix":"10.1111","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4176-6120","authenticated-orcid":false,"given":"Naoki","family":"Mori","sequence":"first","affiliation":[{"name":"Department of Hematology Tokyo Women's Medical University Tokyo 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