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Our objective was to develop a rapid and precise flow cytometric immunobead array (FCIA<\/jats:styled-content>) to quantify VWF<\/jats:styled-content> antigen (VWF<\/jats:styled-content>:Ag) and ristocetin\u2010triggered platelet glycoprotein Ib binding (VWF<\/jats:styled-content>:GPI<\/jats:styled-content>bR) and apply it in a clinical setting.<\/jats:p><\/jats:sec>Methods<\/jats:title>Microbeads, coated with monoclonal antibodies for SZ<\/jats:styled-content>29 or SZ<\/jats:styled-content>151 IgG, were incubated with diluted plasma. VWF<\/jats:styled-content>\u2010binding microbeads were detected with FITC<\/jats:styled-content>\u2010conjugated sheep\u2010anti\u2010human VWF<\/jats:styled-content> IgG by flow cytometry. Plasma VWF<\/jats:styled-content>:Ag and VWF<\/jats:styled-content>:GPI<\/jats:styled-content>bR levels in normal controls (CTL<\/jats:styled-content>; n=105), patients with VWD<\/jats:styled-content> (n=21), and patients with AMI<\/jats:styled-content> (n=146) were tested by FCIA<\/jats:styled-content> and ELISA<\/jats:styled-content> in parallel. ADAMTS<\/jats:styled-content>13 activity and VWF<\/jats:styled-content> multimer analyses were also implemented.<\/jats:p><\/jats:sec>Results<\/jats:title>Our novel FCIA<\/jats:styled-content> showed a strong correlation with the ELISA<\/jats:styled-content> results (VWF<\/jats:styled-content>:Ag, r<\/jats:italic>=.855; VWF<\/jats:styled-content>:GPI<\/jats:styled-content>bR, r<\/jats:italic>=.813). The intra\u2010assay coefficient variations (CV<\/jats:styled-content>s) of VWF<\/jats:styled-content>:Ag\u2010FCIA<\/jats:styled-content> and VWF<\/jats:styled-content>:GPI<\/jats:styled-content>bR\u2010FCIA<\/jats:styled-content> were 9.2% and 7.7%, respectively, and the interassay CV<\/jats:styled-content>s were 12.6% and 13.5%, respectively. Plasma VWF<\/jats:styled-content>:Ag and VWF<\/jats:styled-content>:GPI<\/jats:styled-content>bR levels were significantly higher in patients with AMI<\/jats:styled-content> than in CTL<\/jats:styled-content> (P<\/jats:italic><.0001), whereas the ratios of ADAMTS<\/jats:styled-content>13\/VWF<\/jats:styled-content>:Ag and ADAMTS<\/jats:styled-content>13\/VWF<\/jats:styled-content>:GPI<\/jats:styled-content>bR were significantly lower (P<\/jats:italic><.0001). Levels of plasma ultra\u2010large VWF<\/jats:styled-content> (UL<\/jats:styled-content>\u2010VWF<\/jats:styled-content>) were dramatically increased in patients with AMI<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The novel VWF<\/jats:styled-content>:Ag and VWF<\/jats:styled-content>:GPI<\/jats:styled-content>bR\u2010FCIA<\/jats:styled-content> assays were found to be simpler, more specific, and more accurate than the classical ELISA<\/jats:styled-content> method. In addition, elevated VWF<\/jats:styled-content>:GPI<\/jats:styled-content>bR and UL<\/jats:styled-content>\u2010VWF<\/jats:styled-content> may contribute to the pathogenesis of AMI<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ejh.12905","type":"journal-article","created":{"date-parts":[[2017,5,19]],"date-time":"2017-05-19T04:26:40Z","timestamp":1495168000000},"page":"207-215","source":"Crossref","is-referenced-by-count":5,"title":["Development of a novel flow cytometric immunobead array to quantify VWF: Ag and VWF: GPIbR and its application in acute myocardial infarction"],"prefix":"10.1111","volume":"99","author":[{"given":"Bin","family":"Yan","sequence":"first","affiliation":[{"name":"Jiangsu Institute of Hematology Key Laboratory of Thrombosis and Hemostasis of Ministry of Health The First Affiliated Hospital of Soochow University Suzhou China"},{"name":"Collaborative Innovation Center of Hematology Soochow University Suzhou 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Clinical Medicine Henan University of Science and Technology Luoyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijun","family":"Xia","sequence":"additional","affiliation":[{"name":"Jiangsu Institute of Hematology Key Laboratory of Thrombosis and Hemostasis of Ministry of Health The First Affiliated Hospital of Soochow University Suzhou China"},{"name":"Collaborative Innovation Center of Hematology Soochow University Suzhou China"},{"name":"Cardiovascular Biology Research Program Oklahoma Medical Research Foundation Oklahoma City OK USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changgeng","family":"Ruan","sequence":"additional","affiliation":[{"name":"Jiangsu Institute of Hematology Key Laboratory of Thrombosis and Hemostasis of Ministry of Health The First Affiliated Hospital of Soochow University Suzhou China"},{"name":"Collaborative Innovation Center of Hematology Soochow University Suzhou 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