{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T11:38:59Z","timestamp":1771328339164,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,10,18]],"date-time":"2017-10-18T00:00:00Z","timestamp":1508284800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate the cytokine production and surface marker composition of B cells in adult patients with newly diagnosed primary immune thrombocytopenia (<jats:styled-content style=\"fixed-case\">ITP<\/jats:styled-content>) before and 12\u00a0months after treatment with rituximab + dexamethasone (<jats:styled-content style=\"fixed-case\">RTX<\/jats:styled-content>+<jats:styled-content style=\"fixed-case\">DXM<\/jats:styled-content>) or dexamethasone (<jats:styled-content style=\"fixed-case\">DXM<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Peripheral blood mononuclear cells were isolated from nine patients treated with <jats:styled-content style=\"fixed-case\">RTX<\/jats:styled-content>+<jats:styled-content style=\"fixed-case\">DXM<\/jats:styled-content>, seven patients treated with <jats:styled-content style=\"fixed-case\">DXM<\/jats:styled-content>, and seven healthy donors. Expression of the cell\u2010surface markers <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>5, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>27, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>25, and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>19, and intracellular content of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 were measured by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">PBMC<\/jats:styled-content>s from <jats:styled-content style=\"fixed-case\">ITP<\/jats:styled-content> patients at baseline contained a lower proportion of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010<jats:sup>+<\/jats:sup> B cells (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01) and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106<jats:sup>+<\/jats:sup> B cells (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01) than healthy controls. All patients responded to therapy and levels were normalized at 12\u00a0months. The proportion of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>5<jats:sup>+<\/jats:sup> B cells increased (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01) and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>27<jats:sup>+<\/jats:sup> memory B cells decreased (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05) 12\u00a0months after treatment with <jats:styled-content style=\"fixed-case\">RTX<\/jats:styled-content>+<jats:styled-content style=\"fixed-case\">DXM<\/jats:styled-content> compared to baseline, with an inverse correlation between platelet numbers and the proportion of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>27<jats:sup>+<\/jats:sup> B cells (<jats:italic>R<\/jats:italic>\u00a0=\u00a0\u22120.71; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Both treatment regimens normalized the frequencies of cytokine\u2010producing B cells. The additional increase in <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>5<jats:sup>+<\/jats:sup> B cells after <jats:styled-content style=\"fixed-case\">RTX<\/jats:styled-content>+<jats:styled-content style=\"fixed-case\">DXM<\/jats:styled-content> is compatible with induction of Bregs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ejh.12978","type":"journal-article","created":{"date-parts":[[2017,9,28]],"date-time":"2017-09-28T15:45:55Z","timestamp":1506613555000},"page":"45-52","source":"Crossref","is-referenced-by-count":15,"title":["Effects of rituximab and dexamethasone on regulatory and proinflammatory B\u2010cell subsets in patients with primary immune thrombocytopenia"],"prefix":"10.1111","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0662-3335","authenticated-orcid":false,"given":"Sif","family":"Gudbrandsdottir","sequence":"first","affiliation":[{"name":"Institute for Inflammation Research Center for Rheumatology and Spine Diseases Copenhagen 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H","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Institute for Inflammation Research Center for Rheumatology and Spine Diseases Copenhagen University Hospital Rigshospitalet  Copenhagen 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