{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:06:15Z","timestamp":1790910375916,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Molecular monitoring of treatment response in patients with chronic myelogenous leukemia is performed using the Europe Against Cancer (EAC) qPCR assay using the International Scale (IS). The assay amplifies both e13a2 and e14a2 <jats:italic>BCR\u2010ABL1<\/jats:italic> transcript variants. Observing distinct variant\u2010dependent amplification curves during qPCR, we aimed to determine if this affected quantitation of <jats:italic>BCR\u2010ABL1.<\/jats:italic><\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We investigated the qPCR efficiency at three Danish diagnostic centers (Zealand University Hospital [ZUH], Aarhus University Hospital [AU], and Rigshospitalet [RH]) on cell lines expressing either the e13a2 or e14a2 <jats:italic>BCR\u2010ABL1<\/jats:italic> transcript variants and compared %IS values from 219 chronic myeloid leukemia patients from the centers with either the e13a2 (n\u00a0=\u00a0113) or e14a2 (n\u00a0=\u00a0106) transcript variants obtained by qPCR with absolute quantitation by droplet digital PCR (ddPCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Although no significant differences were found in amplification efficiencies of the transcript variants, Bland\u2010Altman analysis of qPCR vs ddPCR values for patient samples revealed a significant average difference in the bias between variants (e3a2\/e14a2) of 4.6\u2010, 6.5\u2010, and 1.8\u2010fold for ZUH, AU, and RH, respectively. Furthermore, qPCR %IS values of diagnostic patient samples revealed a significant 4.7\u2010fold difference between the e13a2 and e14a2 variants.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings suggest that the EAC qPCR assay may underestimate the e14a2 variant compared to the e13a2 variant.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ejh.13238","type":"journal-article","created":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T14:22:16Z","timestamp":1555338136000},"page":"26-34","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Variant\u2010specific discrepancy when quantitating <i>BCR\u2010ABL1<\/i> e13a2 and e14a2 transcripts using the Europe Against Cancer qPCR assay"],"prefix":"10.1111","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6767-0226","authenticated-orcid":false,"given":"Lasse","family":"Kjaer","sequence":"first","affiliation":[{"name":"Department of Hematology Zealand University Hospital Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vibe","family":"Skov","sequence":"additional","affiliation":[{"name":"Department of Hematology Zealand University Hospital Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morten T.","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of Clinical Genetics Rigshospitalet Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anni","family":"Aggerholm","sequence":"additional","affiliation":[{"name":"Hemodiagnostic Laboratory Aarhus University Hospital Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Clair","sequence":"additional","affiliation":[{"name":"Plateforme PCR Universit\u00e9 de Montpellier Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"Gniot","sequence":"additional","affiliation":[{"name":"Department of Hematology and Stem Cell Transplantation Poznan University of Medical Sciences Poznan Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Soeby","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Zealand University Hospital Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lene","family":"Udby","sequence":"additional","affiliation":[{"name":"Department of Hematology Zealand University Hospital Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikkel H.","family":"Dorff","sequence":"additional","affiliation":[{"name":"Department of Hematology Zealand University Hospital Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Hasselbalch","sequence":"additional","affiliation":[{"name":"Department of Hematology Zealand University Hospital Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niels","family":"Pallisgaard","sequence":"additional","affiliation":[{"name":"Department of Surgical Pathology Zealand University Hospital Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.10.3343"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-03-144790"},{"issue":"5","key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1182\/blood.V78.5.1155.1155","article-title":"Does the breakpoint within the major breakpoint cluster region (M\u2010bcr) influence the duration of the chronic phase in chronic myeloid leukemia? 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