{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T17:32:17Z","timestamp":1770744737429,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,8,18]],"date-time":"2019-08-18T00:00:00Z","timestamp":1566086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009457","name":"Region Sj\u00e6lland","doi-asserted-by":"publisher","award":["13\u2010000849"],"award-info":[{"award-number":["13\u2010000849"]}],"id":[{"id":"10.13039\/501100009457","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Given a proposed role for PD\u2010L1<jats:sup>+<\/jats:sup> and IL\u201010\u2010producing B\u2010cell subsets in promoting certain cancers, we sought to characterize the frequency and phenotype of B cells in patients with chronic myeloproliferative neoplasms (MPNs) and the influence of ruxolitinib and interferon\u2010\u03b12 therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We analyzed B\u2010cell frequencies and phenotype in patients with MPNs (n\u00a0=\u00a0107), before and during treatment with ruxolitinib (n\u00a0=\u00a029), interferon\u2010\u03b12 (n\u00a0=\u00a021), or the two drugs in combination (COMBI; n\u00a0=\u00a042) and healthy donors (HDs; n\u00a0=\u00a052) using flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Myelofibrosis patients had lower lymphocyte counts and proportions of B cells than patients with essential thrombocythemia or polycythemia vera and HDs. The B\u2010cell count correlated inversely with <jats:italic>JAK2<\/jats:italic>\u2010V617F allele burden and spleen size and increased after ruxolitinib or COMBI treatment. The proportions of PD\u2010L1<jats:sup>+<\/jats:sup> B cells and PD\u20101<jats:sup>+<\/jats:sup> B cells were significantly higher in patients with myelofibrosis or polycythemia vera than in HDs and decreased during ruxolitinib and COMBI treatment. The proportions of TNF\u2010\u03b1<jats:sup>+<\/jats:sup> and IL\u20106<jats:sup>+<\/jats:sup> B cells were elevated in myelofibrosis patients. The proportion of IL\u20106<jats:sup>+<\/jats:sup> B cells decreased, and the proportion of IL\u201010<jats:sup>+<\/jats:sup> B cells increased during ruxolitinib treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>B\u2010cell frequency and phenotype were altered in MPN patients. Ruxolitinib therapy had marked effects on both frequency and phenotype.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ejh.13292","type":"journal-article","created":{"date-parts":[[2019,7,12]],"date-time":"2019-07-12T05:49:31Z","timestamp":1562910571000},"page":"351-361","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["B\u2010cell frequencies and immunoregulatory phenotypes in myeloproliferative neoplasms: Influence of ruxolitinib, interferon\u2010\u03b12, or combination treatment"],"prefix":"10.1111","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0360-1595","authenticated-orcid":false,"given":"Anders Lindholm","family":"S\u00f8rensen","sequence":"first","affiliation":[{"name":"Institute for Inflammation Research, Center for Rheumatology and Spine Diseases Copenhagen University Hospital Rigshospitalet Copenhagen Denmark"},{"name":"Department of Hematology Zealand University Hospital Roskilde 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