{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T14:48:00Z","timestamp":1788878880096,"version":"build-2803163510"},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,2,22]],"date-time":"2020-02-22T00:00:00Z","timestamp":1582329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008041","name":"Thora og Viggo Groves Mindelegat","doi-asserted-by":"publisher","award":["10000 DKK"],"award-info":[{"award-number":["10000 DKK"]}],"id":[{"id":"10.13039\/501100008041","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007605","name":"Aarhus Universitet","doi-asserted-by":"publisher","award":["120000 DKK"],"award-info":[{"award-number":["120000 DKK"]}],"id":[{"id":"10.13039\/100007605","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Extracellular vesicles (EVs) are important for intercellular signalling in cancer. Tumour\u2010associated macrophages, expressing the haemoglobin\u2010haptoglobin and mannose receptors CD163 and CD206, are crucial for cancer progression. We recently identified CD163 on EVs in the circulation as a fraction of total soluble CD163 (sCD163). Here, we investigated the presence of CD163 and CD206\u2010positive EVs (EV\u2010CD163, EV\u2010CD206) in patients with multiple myeloma (MM).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We enrolled patients with MM (n\u00a0=\u00a032), monoclonal gammopathy of undetermined significance (MGUS) (n\u00a0=\u00a08) and healthy donors (n\u00a0=\u00a016). Plasma protein levels were determined by ELISA before and after vesicle precipitation. Monocytes were examined by flow cytometry, and leucocyte CD163 mRNA by qPCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fractions of EV\u2010CD163 and EV\u2010CD206 were significantly elevated in patients with newly diagnosed MM (median\u00a0=\u00a039.8%, 76.5%, respectively) compared to patients with relapse (15.6%, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.02, 42.5%, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.003), remission (16.9%, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.0001, 25.2%, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.0001), MGUS (17.8%, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01, 33.1%, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.0005) and healthy donors (14.8%, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.0001, 35.5%, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.0001). Whole blood CD163 mRNA did not vary between the groups. The intermediate monocyte subset showed a higher CD163 expression in newly diagnosed patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results indicate that macrophage\u2010derived EVs may play a role in the late phase of malignant progression of MM, and encourage further EV investigations in functional experiments.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ejh.13371","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T16:19:22Z","timestamp":1576772362000},"page":"409-419","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Circulating extracellular vesicle\u2010associated CD163 and CD206 in multiple myeloma"],"prefix":"10.1111","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0264-8495","authenticated-orcid":false,"given":"Sarah L.","family":"Kvorning","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry Aarhus University Hospital Aarhus Denmark"},{"name":"Department of Biomedicine Aarhus University Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlene C.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Aarhus University Hospital Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niels F.","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of Haematology Aarhus University Hospital Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne","family":"Hokland","sequence":"additional","affiliation":[{"name":"Department of Biomedicine Aarhus University Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morten N.","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Aarhus University Hospital Aarhus Denmark"},{"name":"Department of Biomedicine Aarhus University Aarhus Denmark"},{"name":"Department of Haematology Aarhus University Hospital Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7687-5995","authenticated-orcid":false,"given":"Holger J.","family":"M\u00f8ller","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Aarhus University Hospital Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,2,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(14)70442-5"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-12-194241"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa01133202"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2008.259"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2007.07.004"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1256"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-012-1652-6"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2015.191"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.03.014"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0512231"},{"issue":"4","key":"e_1_2_9_12_1","first-page":"588","article-title":"A monocyte\/macrophage antigen recognized by the four antibodies GHI\/61, Ber\u2010MAC3, Ki\u2010M8 and SM4","volume":"75","author":"Pulford K","year":"1992","journal-title":"Immunology"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.22340"},{"issue":"6","key":"e_1_2_9_14_1","first-page":"295","article-title":"A monoclonal antibody to a novel differentiation antigen on human macrophages associated with the down\u2010regulatory phase of the inflammatory process","volume":"55","author":"Zwadlo G","year":"1987","journal-title":"Exp Cell Biol"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.23098"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-03-771261"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12865-019-0323-y"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V85.10.2910.bloodjournal85102910"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.1.378"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2013-0451"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365513.2011.626868"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejh.12296"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2015.06.003"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0410235"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep40286"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.3A1218-500R"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.36.23376"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2016.10.009"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2018.1458809"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404284"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-10-299487"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/003655102760145852"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22995"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/289458"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-10-117"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-017-0528-y"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01433-3"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2015.1008371"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.05.008"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.67.1.97"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2002.03790.x"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-10-646810"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01977"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2015.11.009"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404296"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.4860"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v4.27066"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0019968"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2014.262"},{"key":"e_1_2_9_50_1","volume-title":"Endotext","author":"Feingold KR","year":"2000"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22787"}],"container-title":["European Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fejh.13371","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejh.13371","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ejh.13371","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejh.13371","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T04:25:55Z","timestamp":1693628755000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ejh.13371"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,22]]},"references-count":50,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,5]]}},"alternative-id":["10.1111\/ejh.13371"],"URL":"https:\/\/doi.org\/10.1111\/ejh.13371","archive":["Portico"],"relation":{},"ISSN":["0902-4441","1600-0609"],"issn-type":[{"value":"0902-4441","type":"print"},{"value":"1600-0609","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,22]]},"assertion":[{"value":"2019-10-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}