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The C\u2010terminal SOCS box domain, mediating the degradation of phospho\u2010JAK2, is often affected or even lacking. The analysis of such variants is hampered by the lack of a SOCS1\u2010specific monoclonal antibody recognizing the C\u2010terminus of SOCS1. As this C\u2010terminus is often lost or mutated in B\u2010cell lymphomas, staining with amino\u2010terminal targeting antibodies in a lymphoma setting might be misleading.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>BALB\/c mice were immunized with a truncated SOCS1 C\u2010terminal protein. The supernatant of generated hybridoma cells was screened by ELISA and, immunohistochemically, on formalin\u2010fixed and paraffin\u2010embedded tonsil. After antibody purification by affinity chromatography, epitope mapping and cross\u2010reactivity check followed via substitution scans. SOCS1 protein expression was investigated on cell cultures and cytoblocks of <jats:italic>SOCS1<jats:sub>WT<\/jats:sub><\/jats:italic> stably transfected HEK293T cells, lymphoma cell lines and lymphoid tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Procedures resulted in one monoclonal IgG1 anti\u2010SOCS1 antibody, 424C, that recognizes and strongly binds to the C\u2010terminal region of SOCS1 in immunoblot and immunohistochemistry analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This new anti\u2010SOCS1 monoclonal antibody is a valuable tool to detect SOCS1 expression dependent on an existing SOCS1 box and, therefore, indicating a full\u2010length SOCS1 protein.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ejh.13620","type":"journal-article","created":{"date-parts":[[2021,3,14]],"date-time":"2021-03-14T00:51:58Z","timestamp":1615683118000},"page":"74-80","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A new reliable and highly specific monoclonal antibody to detect the C\u2010terminal region of silencer of cytokine signaling 1"],"prefix":"10.1111","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5863-2305","authenticated-orcid":false,"given":"Stephanie E.","family":"Weissinger","sequence":"first","affiliation":[{"name":"Institute of Pathology University Hospital Ulm Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malena","family":"Zahn","sequence":"additional","affiliation":[{"name":"Institute of Pathology University Hospital Ulm Ulm 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