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Studies on the impact of HU upon the kinetics of the <jats:italic>JAK2V617F<\/jats:italic> allele burden, leukocyte, and platelet counts over time are scarce.<\/jats:p><\/jats:sec><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Using data\u2010driven analysis as a novel tool to model the kinetics of the <jats:italic>JAK2V617F<\/jats:italic> allele burden and blood cell counts over time during treatment with HU.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>Using serial measurements of <jats:italic>JAK2V617F<\/jats:italic> and correlation analysis of routine hematological values (the Hb\u2010concentration, leukocyte count, platelet count, and lactic dehydrogenase), we present a detailed description and analysis of the kinetics of the <jats:italic>JAK2V617F<\/jats:italic>, leukocyte, and platelet counts and lactic dehydrogenase in 27 patients (PV\u00a0=\u00a018; ET\u00a0=\u00a07; PMF\u00a0=\u00a02), who were followed in the Danish randomized trial (DALIAH). To further analyze the <jats:italic>JAK2V617F<\/jats:italic> kinetics, we use a machine learning clustering algorithm to group the response patterns.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Response patterns were highly heterogeneous, with clustering resulting in 3 groups and 3 outliers. In the large majority of patients, HU treatment was initially associated with a modest decline in the <jats:italic>JAK2V617F<\/jats:italic> allele burden in concert with a decline in leukocyte and platelet counts. However, HU did not induce a sustained and continuous decrease in the <jats:italic>JAK2V617F<\/jats:italic> allele burden.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Using data\u2010driven analysis of the <jats:italic>JAK2V617F<\/jats:italic> allele burden, leukocyte, and platelet kinetics during treatment with HU, we have shown that HU does not induce a sustained decrease in the <jats:italic>JAK2V617F<\/jats:italic> allele burden and neither induces sustained normalization of elevated cell counts in MPN patients. Our results may explain why MPN patients during treatment with HU still have a substantially increased risk of thrombosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ejh.13700","type":"journal-article","created":{"date-parts":[[2021,8,19]],"date-time":"2021-08-19T18:59:24Z","timestamp":1629399564000},"page":"624-633","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Data\u2010driven analysis of the kinetics of the <i>JAK2V617F<\/i> allele burden and blood cell counts during hydroxyurea treatment of patients with polycythemia vera, essential thrombocythemia, and primary myelofibrosis"],"prefix":"10.1111","volume":"107","author":[{"given":"Marc J. 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