{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:02:08Z","timestamp":1747184528873,"version":"3.40.5"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,12,15]],"date-time":"2021-12-15T00:00:00Z","timestamp":1639526400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Accumulating studies show that the tumour suppressor <jats:italic>SOCS1<\/jats:italic> is one of the most frequently mutated genes in lymphomas, often affecting the coding sequence of SOCS1 protein. Depending on the type of mutation and lymphoma concerned, <jats:italic>SOCS1<\/jats:italic>\u00a0mutations have different impacts on progression\u2010free and overall survival. Two antibodies binding the N and C terminals of SOCS1 would be a suitable \u2018test pair\u2019 to identify truncated versions of SOCS1. We, therefore, compared the C\u2010terminal antibody 424C with the N\u2010terminal antibody 4H1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>As 424C has already been characterised, we performed a comparative analysis of anti\u2010SOCS1 antibody 4H1 using immunohistochemistry on human tonsil tissue and chamber slides, immunoblots on SOCS1 wildtype and mutated transfected HEK293T cells and lymphoma cell lines and cross\u2010reactivity analysis and epitope mapping with protein microarrays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with 424C, anti\u2010SOCS1 antibody 4H1\u00a0showed various cross\u2010reactions with other proteins resulting in a \u2018pancellular\u2019 immunohistochemical staining pattern in FFPE lymphoid tissue. Like 424C, 4H1 identified SOCS1 wildtype and SOCS1\u00a0mutations in immunoblot experiments but also bound an unknown protein with high intensity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Anti\u2010SOCS1 antibody 4H1\u00a0may be useful in a molecular setting but is disqualified as an immunohistochemical diagnostic tool due to its very broad non\u2010specific binding.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ejh.13730","type":"journal-article","created":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T05:51:33Z","timestamp":1638424293000},"page":"223-231","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Combination of two monoclonal antibodies with SOCS1 N\u2010 and C\u2010terminal binding sites to address SOCS1 status in B cells and B\u2010cell lymphoma"],"prefix":"10.1111","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5863-2305","authenticated-orcid":false,"given":"Stephanie E.","family":"Weissinger","sequence":"first","affiliation":[{"name":"Institute of Pathology University Hospital Ulm  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malena","family":"Zahn","sequence":"additional","affiliation":[{"name":"Institute of Pathology University Hospital Ulm  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf","family":"Marienfeld","sequence":"additional","affiliation":[{"name":"Institute of Pathology University Hospital Ulm  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Tessmer","sequence":"additional","affiliation":[{"name":"German Cancer Research Center (DKFZ) Antibody Unit, Genomics and Proteomics Core Facilities  Heidelberg 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