{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:38:57Z","timestamp":1788914337574,"version":"build-2803163510"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T00:00:00Z","timestamp":1665964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004319","name":"Pfizer","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004319","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010795","name":"Chugai Pharmaceutical","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010795","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016348","name":"Kyowa Kirin Pharmaceutical Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016348","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007723","name":"Takeda Pharmaceuticals U.S.A.","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007723","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004336","name":"Novartis","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004336","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>A proportion of patients with polycythemia vera (PV) and essential thrombocythemia (ET) harbor non\u2010driver mutations associated with poor prognosis. In this study, we analyzed the frequency of non\u2010driver mutations in a large Japanese PV and ET cohort. Furthermore, we studied the relationship of these mutations and prognosis in Japanese patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We enrolled 843 Japanese patients with PV or ET. Non\u2010driver mutations were analyzed by target resequencing using next\u2010generation sequencing. The association of the mutations with the prognosis was estimated using multivariable logistic regression analysis and log\u2010rank test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Non\u2010driver mutations were detected in 31.1% and 24.5% patients with PV and ET, respectively. Among them, <jats:italic>ASXL1<\/jats:italic> mutations were identified as a risk factor for leukemic\/myelofibrotic transformation in PV and ET patients (hazard ratio: 4.68, <jats:italic>p<\/jats:italic>\u00a0=\u00a0.006). The higher\u2010risk groups of the mutation\u2010enhanced international prognostic system (MIPSS)\u2010PV and MIPSS\u2010ET incorporating non\u2010driver mutations exhibited significantly shorter overall survival compared with the low\u2010risk group (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results implicate the importance of studying non\u2010driver mutations for predicting the prognosis and survival of Japanese PV and ET patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ejh.13882","type":"journal-article","created":{"date-parts":[[2022,10,8]],"date-time":"2022-10-08T12:42:50Z","timestamp":1665232970000},"page":"131-136","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Non\u2010driver gene mutation analysis in a large cohort of polycythemia vera and essential thrombocythemia"],"prefix":"10.1111","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1081-0130","authenticated-orcid":false,"given":"Soji","family":"Morishita","sequence":"first","affiliation":[{"name":"Laboratory for the Development of Therapies Against MPN Juntendo University Graduate School of Medicine  Tokyo Japan"},{"name":"Advanced Hematology Juntendo University Graduate School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3374-9814","authenticated-orcid":false,"given":"Yoshinori","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Advanced Hematology Juntendo University Graduate School of Medicine  Tokyo Japan"},{"name":"Department of Hematology Tottori Prefectural Central Hospital  Tottori Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiho","family":"Furuya","sequence":"additional","affiliation":[{"name":"Department of Hematology Juntendo University Graduate School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8386-1871","authenticated-orcid":false,"given":"Yoko","family":"Edahiro","sequence":"additional","affiliation":[{"name":"Advanced Hematology Juntendo University Graduate School of Medicine  Tokyo Japan"},{"name":"Department of Hematology Juntendo University Graduate School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6156-4211","authenticated-orcid":false,"given":"Tomonori","family":"Ochiai","sequence":"additional","affiliation":[{"name":"Department of Hematology Juntendo University Graduate School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuichi","family":"Shirane","sequence":"additional","affiliation":[{"name":"Department of Hematology Juntendo University Graduate School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadaaki","family":"Inano","sequence":"additional","affiliation":[{"name":"Department of Hematology Juntendo University Graduate School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2896-7737","authenticated-orcid":false,"given":"Hajime","family":"Yasuda","sequence":"additional","affiliation":[{"name":"Department of Hematology Juntendo University Graduate School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miki","family":"Ando","sequence":"additional","affiliation":[{"name":"Department of Hematology Juntendo University Graduate School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3502-5000","authenticated-orcid":false,"given":"Marito","family":"Araki","sequence":"additional","affiliation":[{"name":"Laboratory for the Development of Therapies Against MPN Juntendo University Graduate School of Medicine  Tokyo Japan"},{"name":"Advanced Hematology Juntendo University Graduate School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1880-9126","authenticated-orcid":false,"given":"Norio","family":"Komatsu","sequence":"additional","affiliation":[{"name":"Laboratory for the Development of Therapies Against MPN Juntendo University Graduate School of Medicine  Tokyo Japan"},{"name":"Advanced Hematology Juntendo University Graduate School of Medicine  Tokyo Japan"},{"name":"Department of Hematology Juntendo University Graduate School of Medicine  Tokyo Japan"},{"name":"PharmaEssentia Japan K.K.  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1311347"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2016000208"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-020-03054-x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-01-403279"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2013.163"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2017.06.002"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12040934"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.24388"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.16380"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-03-643544"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-19-0152"},{"issue":"4","key":"e_1_2_9_13_1","first-page":"1209","article-title":"CRISPR\/Cas9\u2010mediated ASXL1 mutations in U937 cells disrupt myeloid differentiation","volume":"52","author":"Wu ZJ","year":"2018","journal-title":"Int J Oncol"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-04-845602"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-020-0595-4"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2011.06.001"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.16450"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/bcj.2015.95"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2018.213108"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2018.215582"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-03-530865"}],"container-title":["European Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejh.13882","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ejh.13882","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejh.13882","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T16:34:00Z","timestamp":1692635640000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ejh.13882"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,17]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["10.1111\/ejh.13882"],"URL":"https:\/\/doi.org\/10.1111\/ejh.13882","archive":["Portico"],"relation":{},"ISSN":["0902-4441","1600-0609"],"issn-type":[{"value":"0902-4441","type":"print"},{"value":"1600-0609","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,17]]},"assertion":[{"value":"2022-08-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}