{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T16:23:57Z","timestamp":1782923037686,"version":"3.54.5"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,7,12]],"date-time":"2023-07-12T00:00:00Z","timestamp":1689120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009122","name":"Ministry of Education","doi-asserted-by":"publisher","award":["NRF\u20102020R1I1A3A04037773"],"award-info":[{"award-number":["NRF\u20102020R1I1A3A04037773"]}],"id":[{"id":"10.13039\/100009122","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The prognostic value of the mutation types and dynamics of <jats:italic>FLT3<\/jats:italic>\u2010ITD in acute myeloid leukemia (AML) and other known factors were studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Initial and follow\u2010up samples from 45 AML patients with <jats:italic>FLT3<\/jats:italic>\u2010ITD mutations were analyzed by fragment length analysis, Sanger sequencing, and next\u2010generation sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Some patients (13%) had multiple <jats:italic>FLT3<\/jats:italic>\u2010ITD mutations, and many of them had acute promyelocytic leukemia (APL). <jats:italic>FLT3<\/jats:italic>\u2010ITD mutations were classified according to mutation types, including duplication\u2010only <jats:italic>FLT3<\/jats:italic>\u2010ITD (52%) and <jats:italic>FLT3<\/jats:italic>\u2010ITD with duplications and insertions (dup\u2009+\u2009ins) (48%). The dup\u2009+\u2009ins <jats:italic>FLT3<\/jats:italic>\u2010ITD variant was independently associated with poor prognosis among non\u2010APL patients (odds ratio, 2.92) in addition to <jats:italic>FLT3<\/jats:italic>\u2010ITD with \u226550% variant allele frequency (VAF). The VAFs of <jats:italic>FLT3<\/jats:italic>\u2010ITD were low (median 2.2%) when detected during morphologic complete remission (CR) after conventional chemotherapy; however, in two patients treated with gilteritinib after relapse, the VAFs of <jats:italic>FLT3<\/jats:italic>\u2010ITD were much higher (&gt;95% and 8.1%) in the morphologic CR state.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The type of <jats:italic>FLT3<\/jats:italic>\u2010ITD mutation is important in prognosis, and the dup\u2009+\u2009ins type of <jats:italic>FLT3<\/jats:italic>\u2010ITD can be an indicator of poor prognosis. In addition, the <jats:italic>FLT3<\/jats:italic>\u2010ITD mutation status may unexpectedly not match the morphologic examination results after gilteritinib treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ejh.14044","type":"journal-article","created":{"date-parts":[[2023,7,12]],"date-time":"2023-07-12T08:39:46Z","timestamp":1689151186000},"page":"562-572","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Prognostic value of the mutation types and dynamics of <scp><i>FLT3<\/i><\/scp>\u2010ITD in acute myeloid leukemia"],"prefix":"10.1111","volume":"111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5513-7811","authenticated-orcid":false,"given":"Mosae","family":"Koo","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Chungnam National University College of Medicine, Chungnam National University Hospital  Daejeon Republic of 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