{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T19:36:29Z","timestamp":1773948989342,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T00:00:00Z","timestamp":1694476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Subtotal or total splenectomy are recommended in severe and should be considered in intermediate forms of hereditary spherocytosis (HS). Data on laparoscopic subtotal splenectomy (LSTS) in HS patients are sparse.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty three patients with HS (median age 10.7\u2009years (yrs), range 1.8\u201315.5) underwent LSTS. Baseline and follow\u2010up investigation included haematological parameters, microscopic analysis of pitted erythrocytes (pitE), and B\u2010cell subpopulations assessed by flow cytometry. Results were compared to those of non\u2010splenectomised HS patients, HS patients after total splenectomy (TS), and healthy individuals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After LSTS, haemoglobin levels were normalised in all patients. During median long\u2010term follow\u2010up of 3.9\u2009yrs (range 1.1\u201314.9), only four patients presented mild anaemia. Despite re\u2010growing of the remnant spleen none of the patients required a second surgical intervention. As compared to TS, PitE in LSTS patients were significantly lower and indicated normal to only moderately decreased spleen function. Relative but not absolute IgM memory B\u2010cell counts were reduced in both LSTS and TS patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>LSTS is effective for the treatment of patients with HS. A small remnant spleen is sufficient to provide adequate phagocytic function and to induce a pool of IgM memory B\u2010cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ejh.14077","type":"journal-article","created":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T06:28:48Z","timestamp":1694586528000},"page":"777-786","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Long\u2010term haematological response and maintained immunological function after laparoscopic subtotal splenectomy in patients with hereditary spherocytosis"],"prefix":"10.1111","volume":"111","author":[{"given":"Alica L.","family":"M\u00fcnch","sequence":"first","affiliation":[{"name":"Department of Paediatrics University Medical Center Ulm (UUMC)  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva\u2010Maria","family":"Jacobsen","sequence":"additional","affiliation":[{"name":"Department of Paediatrics University Medical Center Ulm (UUMC)  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ansgar","family":"Schulz","sequence":"additional","affiliation":[{"name":"Department of Paediatrics University Medical Center Ulm (UUMC)  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Loichinger","sequence":"additional","affiliation":[{"name":"Department of Paediatrics University Medical Center Ulm (UUMC)  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias","family":"Wowra","sequence":"additional","affiliation":[{"name":"Department of Paediatrics University Medical Center Ulm (UUMC)  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Schiefele","sequence":"additional","affiliation":[{"name":"Department of Paediatrics University Medical Center Ulm (UUMC)  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Elsner","sequence":"additional","affiliation":[{"name":"Department of Paediatrics University Medical Center Ulm (UUMC)  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mike\u2010Andrew","family":"Westhoff","sequence":"additional","affiliation":[{"name":"Department of Paediatrics University Medical Center Ulm (UUMC)  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandre","family":"Serra","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery UUMC  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriele","family":"Strauss","sequence":"additional","affiliation":[{"name":"Department of Paediatrics Helios Clinic  Berlin\u2010Buch Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"Schaarschmidt","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery Helios Clinic  Berlin\u2010Buch Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6923-488X","authenticated-orcid":false,"given":"Holger","family":"Cario","sequence":"additional","affiliation":[{"name":"Department of Paediatrics University Medical Center Ulm (UUMC)  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,9,12]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-12-512772"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2006.03.005"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12657"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminhematol.2004.01.002"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2016.161166"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2796.1998.00362.x"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2011.08921.x"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1053\/jinf.2001.0904"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2008.03024.x"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)64276-6"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V81.8.2014.2014"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.2.399"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2010.09.090"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.23888"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.SLA.0000048453.61168.8F"},{"issue":"119","key":"e_1_2_11_17_1","first-page":"848","article-title":"Laparoscopic subtotal splenectomy in children and adolescents with spherocytosis","volume":"2022","author":"Le M","year":"2022","journal-title":"Dtsch Arztebl Int"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2010.01.037"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00464-007-9427-1"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1053\/jpsu.2002.31620"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2016.03.005"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2015150887"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-985889"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-010-1049-1"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)61493-6"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.289.6449.861"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/105.5.548"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-11-679357"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1053\/jpsu.2002.35412"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.26592"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.sla.0000150070.69769.39"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-022-00399-x"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-01-0346"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20022020"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023164"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxs050"}],"container-title":["European Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejh.14077","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,6]],"date-time":"2024-01-06T08:47:15Z","timestamp":1704530835000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ejh.14077"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,12]]},"references-count":35,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,11]]}},"alternative-id":["10.1111\/ejh.14077"],"URL":"https:\/\/doi.org\/10.1111\/ejh.14077","archive":["Portico"],"relation":{},"ISSN":["0902-4441","1600-0609"],"issn-type":[{"value":"0902-4441","type":"print"},{"value":"1600-0609","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,12]]},"assertion":[{"value":"2023-06-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}