{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:08:04Z","timestamp":1790402884059,"version":"4.1.0"},"reference-count":107,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T00:00:00Z","timestamp":1693180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The Philadelphia\u2010negative chronic myeloproliferative neoplasms (MPNs) are associated with a huge comorbidity burden, including an increased risk of cardiovascular diseases. Recently, chronic inflammation has been suggested to be the driving force for clonal evolution and disease progression in MPN but also potentially having an impact upon the development of accelerated (premature) atherosclerosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Since chronic inflammation, atherosclerosis, and atherothrombosis are prevalent in MPNs and we have previously shown oxidative stress genes to be markedly upregulated in MPNs, we hypothesized that genes linked to development of atherosclerosis might be highly deregulated as well.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using whole blood gene expression profiling in patients with essential thrombocythemia (ET; <jats:italic>n<\/jats:italic>\u2009=\u200919), polycythemia vera (PV; <jats:italic>n<\/jats:italic>\u2009=\u200941), or primary myelofibrosis (PMF; <jats:italic>n<\/jats:italic>\u2009=\u20099), we herein for the first time report aberrant expression of several atherosclerosis genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of 84 atherosclerosis genes, 45, 56, and 46 genes were deregulated in patients with ET, PV, or PMF, respectively. Furthermore, <jats:italic>BCL2L1, MMP1, PDGFA, PTGS1<\/jats:italic>, and <jats:italic>THBS4<\/jats:italic> were progressively significantly upregulated and <jats:italic>BCL2<\/jats:italic> progressively significantly downregulated from ET over PV to PMF (all FDR &lt;0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We have for the first time shown massive deregulation of atherosclerosis genes in MPNs, likely reflecting the inflammatory state in MPNs in association with in vivo activation of leukocytes, platelets, and endothelial cells being deeply involved in the atherosclerotic process.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ejh.14081","type":"journal-article","created":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T00:13:40Z","timestamp":1693268020000},"page":"805-814","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Whole blood transcriptional profiling reveals highly deregulated atherosclerosis genes in Philadelphia\u2010chromosome negative myeloproliferative neoplasms"],"prefix":"10.1111","volume":"111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0097-7826","authenticated-orcid":false,"given":"Vibe","family":"Skov","sequence":"first","affiliation":[{"name":"Department of Hematology Zealand University Hospital  Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mads","family":"Thomassen","sequence":"additional","affiliation":[{"name":"Department of Clinical Genetics Odense University Hospital  Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6767-0226","authenticated-orcid":false,"given":"Lasse","family":"Kj\u00e6r","sequence":"additional","affiliation":[{"name":"Department of Hematology Zealand University Hospital  Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morten Kranker","family":"Larsen","sequence":"additional","affiliation":[{"name":"Department of Hematology Zealand University Hospital  Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trine A.","family":"Knudsen","sequence":"additional","affiliation":[{"name":"Department of Hematology Zealand University Hospital  Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Ellervik","sequence":"additional","affiliation":[{"name":"Department of Pathology Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torben A.","family":"Kruse","sequence":"additional","affiliation":[{"name":"Department of Clinical Genetics Odense University Hospital  Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3936-8032","authenticated-orcid":false,"given":"Hans Carl","family":"Hasselbalch","sequence":"additional","affiliation":[{"name":"Department of Hematology Zealand University Hospital  Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra063728"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1406186"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/102476"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejim.2015.03.009"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.2147\/CLEP.S216787"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2020008109"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2020008043"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2011.055392"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2010.031070"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-11-394775"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2012.10.020"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2013.01.004"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/964613"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/14529"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/606819"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/284706"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2015.08.014"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2016.12"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2017.06.010"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0183620"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045\u2010017\u20100425\u2010z"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2017.06.004"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers10040104"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11899\u2010019\u201000508\u2010w"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12082250"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12082038"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9082334"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9111037"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2020.100711"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2020.11.003"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2013.102"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/648090"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2020003291"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1408617"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1409405"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-03-631747"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1701719"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCGEN.118.001926"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2018.1.264"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.312115"},{"issue":"3","key":"e_1_2_10_42_1","first-page":"133","article-title":"Atherosclerosis and clonal hematopoiesis: a new risk factor","volume":"30","author":"P\u00e1ramo Fern\u00e1ndez JA","year":"2018","journal-title":"Clin Investig Arterioscler"},{"issue":"854","key":"e_1_2_10_43_1","first-page":"1","article-title":"Roles of JAK2 in aging, inflammation, hematopoiesis and malignant transformation","volume":"8","author":"Perner F","year":"2019","journal-title":"Cell"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27752"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathmechdis-012419-032544"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-019-0247-5"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21217902"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2020.02.008"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpc.2020.100130"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20200306"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/hvaa275"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/imj.13407"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2020.264085"},{"issue":"1","key":"e_1_2_10_54_1","first-page":"39","article-title":"Essential thrombocythemia and aortic dissection, causal or incidental association?","volume":"47","author":"Laktib N","year":"2022","journal-title":"J Med Vasc"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2022.06.029"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0112786"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.2307\/1390807"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1093\/biostatistics\/4.2.249"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199408183310709"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199901143400207"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-018-0064-0"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00586"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-019-0675-0"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495\u2010006\u20109402\u20107"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2020.277111"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/9454501"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaccao.2022.04.002"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2012.07.100"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2010.07.005"},{"key":"e_1_2_10_70_1","first-page":"2860","article-title":"Increased oxidative stress in patients with essential thrombocythemia","volume":"17","author":"Durmus A","year":"2013","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2021.101895"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11010113"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9149(02)03144-2"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2004.01373.x"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1080\/10715760500539154"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000150649.39934.13"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.287.19.2570"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.108.182642"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.46.11.1853"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajh\/6.11.314S"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000148548.47755.22"},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2013.03.011"},{"key":"e_1_2_10_83_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(00)00647-1"},{"key":"e_1_2_10_84_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3026(22)00116-8"},{"key":"e_1_2_10_85_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010019\u20102203\u2010z"},{"key":"e_1_2_10_86_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.pmbts.2017.02.005"},{"key":"e_1_2_10_87_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25430"},{"key":"e_1_2_10_88_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2004.03.004"},{"key":"e_1_2_10_89_1","doi-asserted-by":"publisher","DOI":"10.1159\/000049716"},{"key":"e_1_2_10_90_1","doi-asserted-by":"publisher","DOI":"10.1038\/clpt.2009.108"},{"key":"e_1_2_10_91_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms16034744"},{"key":"e_1_2_10_92_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-03-460154"},{"key":"e_1_2_10_93_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvab003"},{"key":"e_1_2_10_94_1","doi-asserted-by":"publisher","DOI":"10.7326\/M17-0028"},{"key":"e_1_2_10_95_1","doi-asserted-by":"publisher","DOI":"10.1002\/rth2.12657"},{"key":"e_1_2_10_96_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.17.1.25"},{"key":"e_1_2_10_97_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2017.140"},{"key":"e_1_2_10_98_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12092533"},{"key":"e_1_2_10_99_1","doi-asserted-by":"publisher","DOI":"10.1159\/000438511"},{"key":"e_1_2_10_100_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2022.845942"},{"key":"e_1_2_10_101_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep05519"},{"key":"e_1_2_10_102_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.110.988915"},{"key":"e_1_2_10_103_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.105.534107"},{"key":"e_1_2_10_104_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.105.547414"},{"key":"e_1_2_10_105_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.24.14367"},{"key":"e_1_2_10_106_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-04-348755"},{"key":"e_1_2_10_107_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2352\u20103026(15)00092\u20107"},{"key":"e_1_2_10_108_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201141727"}],"container-title":["European Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejh.14081","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,6]],"date-time":"2024-01-06T08:47:52Z","timestamp":1704530872000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ejh.14081"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,28]]},"references-count":107,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,11]]}},"alternative-id":["10.1111\/ejh.14081"],"URL":"https:\/\/doi.org\/10.1111\/ejh.14081","archive":["Portico"],"relation":{},"ISSN":["0902-4441","1600-0609"],"issn-type":[{"value":"0902-4441","type":"print"},{"value":"1600-0609","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,28]]},"assertion":[{"value":"2023-06-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}