{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T13:02:00Z","timestamp":1771506120724,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T00:00:00Z","timestamp":1711497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100012021","name":"Iran University of Medical Sciences","doi-asserted-by":"publisher","award":["16492"],"award-info":[{"award-number":["16492"]}],"id":[{"id":"10.13039\/100012021","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Recent studies scrutinize how NETosis (a unique cell death mechanism of neutrophil), impacts thrombosis patients with essential thrombocythemia (ET). This research evaluates the susceptibility of ET neutrophils to form NETs and tests two potential inhibitors, resveratrol (RSV) and tetrahydroisoquinoline (THIQ), in vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Platelet\u2010rich plasma from low\u2010risk ET patients was used, along with neutrophils from both patients and controls. NET formation assays, with or without RSV and THIQ treatment after LPS stimulation, were conducted in a CO<jats:sub>2<\/jats:sub> incubator. Evaluation included flow cytometry and fluorescence microscopy for NET formation and ELISA for TNF\u03b1, IL8, and vWF:Ag levels in patient and control plasma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Neutrophils from ET patients released more NETs than controls, confirmed by flow cytometry and fluorescence microscopy. Additionally, patients had significantly higher plasma levels of IL8 and TNF\u03b1 compared to controls, while RSV was more effective than THIQ in reducing NETosis rates in these patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In ET patients, a platelet counts over 1 million indicates the need for preventive treatment against thrombotic events. Similarly, in this study, RSV and THIQ significantly reduced the rate of NETosis in ET patients with higher platelet counts, and this role was more prominent in the case of the second inhibitor (RSV).<\/jats:p><\/jats:sec>","DOI":"10.1111\/ejh.14196","type":"journal-article","created":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T06:14:47Z","timestamp":1711606487000},"page":"44-53","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Resveratrol and tetrahydroisoquinoline effects on neutrophil sensitivity to <scp>NETosis<\/scp> formation in low\u2010risk essential thrombocythemia patients"],"prefix":"10.1111","volume":"113","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-2814-3555","authenticated-orcid":false,"given":"Fahimeh","family":"Shahriyary","sequence":"first","affiliation":[{"name":"Department of Hematology and Blood Banking, School of Allied Medical Sciences Iran University of Medical Sciences  Tehran 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