{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T15:22:59Z","timestamp":1788880979339,"version":"build-2803163510"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T00:00:00Z","timestamp":1748908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T00:00:00Z","timestamp":1748908800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100017959","name":"Health Commission of Hebei Province","doi-asserted-by":"publisher","award":["20251054"],"award-info":[{"award-number":["20251054"]}],"id":[{"id":"10.13039\/100017959","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Adult acute leukemia is a common hematopoietic malignancy. Current diagnostic methods have limitations, and there is a need for non\u2010invasive early diagnosis and follow\u2010up monitoring. This study aimed to evaluate the diagnostic and prognostic significance of peripheral blood RASSF1A, P16, and PTGER4 gene methylation in adult acute leukemia. From January 2023 to December 2024, 78 adult acute leukemia patients and 25 controls were enrolled. Peripheral blood samples were collected before chemotherapy and 30\u2009days after initial treatment for gene methylation analysis. The results showed that the combined detection of these three genes had a diagnostic sensitivity of 82.1% and a specificity of 92.0%. Positive methylation was significantly associated with a higher proportion of bone marrow immature cells, chromosomal karyotype abnormalities, and a greater likelihood of bone marrow transplantation. After 30\u2009days of initial treatment, peripheral blood gene methylation levels decreased significantly. In conclusion, the combined detection of peripheral blood gene methylation can identify high\u2010risk acute leukemia patients, serve as a prognostic marker, and be used for post\u2010treatment follow\u2010up monitoring.<\/jats:p>","DOI":"10.1111\/ejh.14442","type":"journal-article","created":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T21:59:48Z","timestamp":1748987988000},"page":"653-661","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Diagnostic and Prognostic Significance of Peripheral Blood\n                    <scp>RASSF1A<\/scp>\n                    ,\n                    <scp>P16,<\/scp>\n                    and\n                    <scp>PTGER4<\/scp>\n                    Gene Methylation in Adult Acute Leukemia"],"prefix":"10.1111","volume":"117","author":[{"given":"Ya","family":"Li","sequence":"first","affiliation":[{"name":"Department of Endocrinology The 980th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Hematology The Second Hospital of Hebei Medical University  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hematology The 980th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanfen","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Hematology The 980th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanlei","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy The 980th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyuan","family":"Tian","sequence":"additional","affiliation":[{"name":"Fourth Outpatient Department The 980th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5636-4585","authenticated-orcid":false,"given":"Tianhua","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Hematology The 980th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,3]]},"reference":[{"issue":"1","key":"e_1_2_8_2_1","first-page":"27","article-title":"Promoter Methylation of p16 and EDNRB Gene in Leukemia Patients in Taiwan","volume":"51","author":"Hsiao P. 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