{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T18:17:22Z","timestamp":1780424242153,"version":"3.54.1"},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T00:00:00Z","timestamp":1755043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013924","name":"Wirtschaftsagentur Wien","doi-asserted-by":"publisher","award":["2841342"],"award-info":[{"award-number":["2841342"]}],"id":[{"id":"10.13039\/501100013924","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      Pediatric AML with\n                      <jats:italic>KMT2A::MLLT10<\/jats:italic>\n                      accounts for 10%\u201315% of\n                      <jats:italic>KMT2A<\/jats:italic>\n                      \u2010rearranged AML and is associated with poor prognosis. Lately, the assessment of measurable residual disease (MRD) by reverse transcription quantitative polymerase chain reaction (RT\u2010qPCR) has become an important tool for disease management; however, in the pediatric setting, it lacks standardized protocols. Therefore, we investigated the prognostic relevance of MRD monitoring by RT\u2010qPCR during high\u2010dose polychemotherapy in pediatric patients with AML expressing\n                      <jats:italic>KMT2A::MLLT10<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Using RNA sequencing, we determined the fusion breakpoints and designed RT\u2010qPCR assays for MRD monitoring. Bone marrow samples collected from 41 patients, who were treated in the AML\u2010BFM or AIEOP study, were analyzed for MRD by RT\u2010qPCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      MRD positivity after the second treatment course resulted in a significantly worse probability of overall survival (pOS) compared to MRD negative patients (33.3%\u2009\u00b1\u200919.2% vs. 80.6%\u2009\u00b1\u20097.8%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.032). Moreover, the probability of event\u2010free survival (pEFS) (16.7%\u2009\u00b1\u200915.2% vs. 76.9%\u2009\u00b1\u20098.3%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.003) and cumulative incidence of relapse (CIR) (83.3%\u2009\u00b1\u200940.8% vs. 19.2%\u2009\u00b1\u200940.2%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.001) were significantly worse for patients in complete morphologic remission who remained MRD positive after the second treatment course.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Thus, MRD monitoring enables the identification of a subgroup of pediatric patients with AML carrying\n                      <jats:italic>KMT2A::MLLT10<\/jats:italic>\n                      in complete morphologic remission with a dismal prognosis despite the current intensive therapy regimen.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Trial Registration<\/jats:title>\n                    <jats:p>\n                      AML\u2010BFM study 2004:\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/clinicaltrials.gov\">ClinicalTrials.gov<\/jats:ext-link>\n                      Identifier: NCT00111345; AML\u2010BFM registry 2012 and AML\u2010BFM study 2012: EudraCT 2013\u2010000018\u201039; AML\u2010BFM registry 2017: DRKS number: DRKS00013030\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ejh.70019","type":"journal-article","created":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T23:10:15Z","timestamp":1755126615000},"page":"493-504","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Prognostic Value of Molecular Genetic Measurable Residual Disease (\n                    <scp>MRD<\/scp>\n                    ) Monitoring in Pediatric Acute Myeloid Leukemia Expressing\n                    <i>KMT2A::MLLT10<\/i>"],"prefix":"10.1111","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-8456-4716","authenticated-orcid":false,"given":"Emma","family":"Steidel","sequence":"first","affiliation":[{"name":"Department of Pediatric Hematology\u2010Oncology, Pediatrics III University Hospital of Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eser","family":"Orhan","sequence":"additional","affiliation":[{"name":"Pediatric Research Network  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mareike","family":"Rasche","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology\u2010Oncology, Pediatrics III University Hospital of Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Pigazzi","sequence":"additional","affiliation":[{"name":"Division of Pediatric Hematology, Oncology and Stem Cell Transplant, Department of Women's and Children's Health University of Padua  Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Tregnago","sequence":"additional","affiliation":[{"name":"Division of Pediatric Hematology, Oncology and Stem Cell Transplant, Department of Women's and Children's Health University of Padua  Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina 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