{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T05:25:45Z","timestamp":1783574745307,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T00:00:00Z","timestamp":1757376000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["24ek0510046h0001"],"award-info":[{"award-number":["24ek0510046h0001"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K11515"],"award-info":[{"award-number":["24K11515"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Polymerase chain reaction (PCR)\u2010based Minimal residual disease (MRD) detection is commonly used for core\u2010binding factor acute myeloid leukemia (CBF\u2010AML), but its interpretation in the context of allogeneic hematopoietic stem cell transplantation (allo\u2010HSCT) remains under discussion.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>Using Kyoto Stem Cell Transplantation Group registry data, we included 96 patients who underwent allo\u2010HSCT between 2000 and 2019 for CBF\u2010AML.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      To assess MRD, quantitative PCR with\n                      <jats:italic>GAPDH<\/jats:italic>\n                      control was most used. As for pretransplant MRD, 2\u2010year overall survival (OS) and relapse\u2010free survival (RFS) of the weak positive group (50\u2013200 copy\/\u03bcgRNA) were 66.5% and 67.2%, and these were as unfavorable as those of the strong positive group (&gt;\u2009200 copy\/\u03bcgRNA, OS; 64.3%, RFS; 54.5%) compared to the negative group (OS; 100%, RFS; 93.3%). In contrast, as regards posttransplant MRD, 2\u2010year OS of patients with lower maximum MRD during approximately half a year post\u2010transplantation period (50\u2013350 copy\/\u03bcgRNA, 81.5%) was comparable to that of MRD\u2010negative patients (84.5%). In multivariate analysis, higher maximum MRD (&gt;\u2009350 copy\/\u03bcgRNA) was a significant unfavorable factor toward OS (hazard ratio: 6.3,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.002) compared to negative MRD.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The interpretation of posttransplant MRD positivity should be approached with caution, particularly in cases with low\u2010level positivity as it might diminish over time.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ejh.70024","type":"journal-article","created":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T07:55:56Z","timestamp":1757404556000},"page":"574-583","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Significance of Pre\u2010 and Posttransplant Minimal Residual Disease on Transplant Outcomes in Core\u2010Binding Factor Acute Myeloid Leukemia"],"prefix":"10.1111","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1701-7946","authenticated-orcid":false,"given":"Chika","family":"Tokuda","sequence":"first","affiliation":[{"name":"Department of Hematology, Graduate School of Medicine Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Iwasaki","sequence":"additional","affiliation":[{"name":"Department of Hematology, Graduate School of Medicine Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junya","family":"Kanda","sequence":"additional","affiliation":[{"name":"Department of Hematology, Graduate School of Medicine Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadakazu","family":"Kondo","sequence":"additional","affiliation":[{"name":"Department of Hematology, Graduate School of Medicine Kyoto University  Kyoto Japan"},{"name":"Department of Hematology Kobe City Medical Center General Hospital  Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuyuki","family":"Arai","sequence":"additional","affiliation":[{"name":"Department of Hematology, Graduate School of Medicine Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Department of Hematology Kobe City Medical Center General Hospital  Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryusuke","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Hematology Kobe City Medical Center General Hospital  Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazunori","family":"Imada","sequence":"additional","affiliation":[{"name":"Department of Hematology Japanese Red Cross Osaka Hospital  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Oku","sequence":"additional","affiliation":[{"name":"Department of Hematology, Graduate School of Medicine Kyoto University  Kyoto Japan"},{"name":"Department of Hematology Japanese Red Cross Osaka Hospital  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4487-5808","authenticated-orcid":false,"given":"Yasunori","family":"Ueda","sequence":"additional","affiliation":[{"name":"Department of Hematology\/Oncology Kurashiki Central Hospital  Kurashiki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryosuke","family":"Takatani","sequence":"additional","affiliation":[{"name":"Department of Hematology\/Oncology Kurashiki Central Hospital  Kurashiki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihito","family":"Yonezawa","sequence":"additional","affiliation":[{"name":"Department of Hematology Kokura Memorial Hospital  Kitakyushu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumie","family":"Iwai","sequence":"additional","affiliation":[{"name":"Department of Hematology Kokura Memorial Hospital  Kitakyushu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Yago","sequence":"additional","affiliation":[{"name":"Department of Hematology Shizuoka General Hospital  Shizuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Ikeda","sequence":"additional","affiliation":[{"name":"Division of Hematology and Stem Cell Transplantation Shizuoka Cancer Center  Shizuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanako","family":"Yoshitsugu","sequence":"additional","affiliation":[{"name":"Division of Hematology and Stem Cell Transplantation Shizuoka Cancer Center  Shizuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiyuki","family":"Kitano","sequence":"additional","affiliation":[{"name":"Department of Hematology, Kitano Hospital Tazuke Kofukai Medical Research Institute  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Akasaka","sequence":"additional","affiliation":[{"name":"Department of Hematology Tenri Hospital  Tenri Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaharu","family":"Nohgawa","sequence":"additional","affiliation":[{"name":"Department of Hematology Japanese Red Cross Wakayama Medical Center  Wakayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiro","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Hematology Japanese Red Cross Wakayama Medical Center  Wakayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuru","family":"Itoh","sequence":"additional","affiliation":[{"name":"Department of Hematology Kyoto City Hospital  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoharu","family":"Takeoka","sequence":"additional","affiliation":[{"name":"Department of Hematology and Immunology Japanese Red Cross Otsu Hospital  Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoyuki","family":"Anzai","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Takatsuki Red Cross Hospital  Takatsuki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyuki","family":"Onda","sequence":"additional","affiliation":[{"name":"Department of Hematology, Graduate School of Medicine Kyoto University  Kyoto Japan"},{"name":"Department of Hematology and Oncology Takatsuki Red Cross Hospital  Takatsuki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuyoshi","family":"Arima","sequence":"additional","affiliation":[{"name":"Department of Hematology Shinko Hospital  Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Tsunemine","sequence":"additional","affiliation":[{"name":"Department of Hematology Shinko Hospital  Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masakatsu","family":"Hishizawa","sequence":"additional","affiliation":[{"name":"Department of Hematology Kyoto\u2010Katsura Hospital  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsumasa","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Hematology Hyogo Prefectural Amagasaki General Medical Center  Amagasaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirokazu","family":"Hirata","sequence":"additional","affiliation":[{"name":"Department of Hematology Kansai Electric Power Hospital  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kosuke","family":"Asagoe","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Shiga General Hospital  Shiga Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6508-9432","authenticated-orcid":false,"given":"Noriyoshi","family":"Yoshinaga","sequence":"additional","affiliation":[{"name":"Department of Hematology, Graduate School of Medicine Kyoto University  Kyoto Japan"},{"name":"Department of Hematology and Oncology Shiga General Hospital  Shiga Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Department of Hematology, Graduate School of Medicine Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kouhei","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Department of Hematology, Graduate School of Medicine Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akifumi","family":"Takaori\u2010Kondo","sequence":"additional","affiliation":[{"name":"Department of Hematology, Graduate School of Medicine Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Kyoto Stem Cell Transplantation Group (KSCTG)","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,9]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2021013626"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V88.6.2183.bloodjournal8862183"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.11.4789.bloodjournal87114789"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1995.tb05253.x"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.13.7521"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.32769"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/sim.4509"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2009.07.004"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2012.244"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.14340"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-021-04494-z"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejh.13089"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402478"},{"issue":"9","key":"e_1_2_11_15_1","first-page":"1082","article-title":"The Influence of Age on Prognosis of De Novo Acute Myeloid Leukemia Differs According to Cytogenetic Subgroups","volume":"89","author":"Schoch C.","year":"2004","journal-title":"Haematologica"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402159"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.2.443"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2003.03.166"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404015"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2010.28.6468"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-06-435669"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-018-0158-1"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2019000709"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2006.06276.x"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hemonc.2016.08.005"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2021.278645"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-017-0414-2"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-05-1357"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12253-015-0002-0"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-020-04051-0"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70127-3"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2015.10.006"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.203"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.14740\/wjon1661"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-12-688705"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-10-462879"}],"container-title":["European Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejh.70024","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T01:50:57Z","timestamp":1762221057000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ejh.70024"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,9]]},"references-count":35,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1111\/ejh.70024"],"URL":"https:\/\/doi.org\/10.1111\/ejh.70024","archive":["Portico"],"relation":{},"ISSN":["0902-4441","1600-0609"],"issn-type":[{"value":"0902-4441","type":"print"},{"value":"1600-0609","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,9]]},"assertion":[{"value":"2025-05-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}