{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T01:53:57Z","timestamp":1762221237279,"version":"build-2065373602"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T00:00:00Z","timestamp":1757289600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006426","name":"Eva og Henry Fr\u00e6nkels Mindefond","doi-asserted-by":"publisher","award":["2023\u20100115"],"award-info":[{"award-number":["2023\u20100115"]}],"id":[{"id":"10.13039\/501100006426","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Clonotyping of immunoglobulin heavy chain (IGH) gene rearrangements is critical for diagnosis, prognostication, and measurable residual disease monitoring in chronic lymphocytic leukemia (CLL). Although short\u2010read next\u2010generation sequencing (NGS) platforms, such as Illumina MiSeq, are widely used, they face challenges in spanning full VDJ rearrangements. Long\u2010read sequencing via Oxford Nanopore Technologies (ONT) offers a potential alternative using the compact and cost\u2010effective flow cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We evaluated IGH clonotyping in samples from 13 CLL patients using ONT MinION with Flongle flow cells and super\u2010accuracy GPU\u2010based base calling (Dorado), comparing results to MiSeq\u2010based LymphoTrack assays. Amplicons were generated using the IGH FR1 assay and analyzed using Smith\u2010Waterman alignment and IgBlast tools.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      All major and minor clonotypes identified by MiSeq were matched with 100% sequence identity using Nanopore sequencing. Clonal burden estimates were strongly correlated (Pearson\n                      <jats:italic>\u03c1<\/jats:italic>\n                      \u2009=\u20090.87,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u200910\n                      <jats:sup>\u22124<\/jats:sup>\n                      ), although with considerable variability. Somatic hypermutation status was reliably assessed with super\u2010accuracy base calling (Q30: 76%). Fast or CPU\u2010only base calling was insufficient for accurate mutation analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Nanopore sequencing enables accurate and rapid IGH clonotyping in CLL, offering comparable performance to MiSeq with lower cost and laboratory footprint. This supports its potential utility in routine and decentralized hematopathology workflows.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ejh.70030","type":"journal-article","created":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T06:11:22Z","timestamp":1757398282000},"page":"584-590","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Performance of Rapid Clonotyping of Chronic Lymphocytic Leukemia Using Flongle Flow\u2010Cell Nanopore Sequencing of\n                    <scp>IGH<\/scp>\n                    Rearrangements"],"prefix":"10.1111","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3083-4850","authenticated-orcid":false,"given":"Marcus H\u00f8y","family":"Hansen","sequence":"first","affiliation":[{"name":"Haematology\u2010Pathology Research Laboratory, Research Unit for Haematology and Research Unit for Pathology University of Southern Denmark and Odense University Hospital  Odense Denmark"},{"name":"CITCO, Centre for Cellular Immunotherapy of Haematological Cancer Odense Odense University Hospital  Odense Denmark"},{"name":"Department of Haematology Odense University Hospital  Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rona","family":"Gulmir","sequence":"additional","affiliation":[{"name":"Haematology\u2010Pathology Research Laboratory, Research Unit for Haematology and Research Unit for Pathology University of Southern Denmark and Odense University Hospital  Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6632-8039","authenticated-orcid":false,"given":"Oriane","family":"C\u00e9dile","sequence":"additional","affiliation":[{"name":"Haematology\u2010Pathology Research Laboratory, Research Unit for Haematology and Research Unit for Pathology University of Southern Denmark and Odense University Hospital  Odense Denmark"},{"name":"CITCO, Centre for Cellular Immunotherapy of Haematological Cancer Odense Odense University Hospital  Odense Denmark"},{"name":"Department of Haematology Odense University Hospital  Odense Denmark"},{"name":"OPEN, Odense Patient Data Explorative Network Odense University Hospital  Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6427-4276","authenticated-orcid":false,"given":"Sara Kamuk","family":"Dahlmann","sequence":"additional","affiliation":[{"name":"Haematology\u2010Pathology Research Laboratory, Research Unit for Haematology and Research Unit for Pathology University of Southern Denmark and Odense University Hospital  Odense Denmark"},{"name":"CITCO, Centre for Cellular Immunotherapy of Haematological Cancer Odense Odense University Hospital  Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5018-3728","authenticated-orcid":false,"given":"S\u00f3lja Remisd\u00f3ttir","family":"Veyhe","sequence":"additional","affiliation":[{"name":"Haematology\u2010Pathology Research Laboratory, Research Unit for Haematology and Research Unit for Pathology University of Southern Denmark and Odense University Hospital  Odense Denmark"},{"name":"CITCO, Centre for Cellular Immunotherapy of Haematological Cancer Odense Odense University Hospital  Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7456-902X","authenticated-orcid":false,"given":"Magnus","family":"Stougaard","sequence":"additional","affiliation":[{"name":"Department for Clinical Genetics, Department of Clinical Medicine Aarhus University and Aarhus University Hospital  Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2158-0107","authenticated-orcid":false,"given":"Karen","family":"Juul\u2010Jensen","sequence":"additional","affiliation":[{"name":"Haematology\u2010Pathology Research Laboratory, Research Unit for Haematology and Research Unit for Pathology University of Southern Denmark and Odense University Hospital  Odense Denmark"},{"name":"Department of Haematology Odense University Hospital  Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0411-7594","authenticated-orcid":false,"given":"Charlotte Guldborg","family":"Nyvold","sequence":"additional","affiliation":[{"name":"Haematology\u2010Pathology Research Laboratory, Research Unit for Haematology and Research Unit for Pathology University of Southern Denmark and Odense University Hospital  Odense Denmark"},{"name":"CITCO, Centre for Cellular Immunotherapy of Haematological Cancer Odense Odense University Hospital  Odense Denmark"},{"name":"Department of Haematology Odense University Hospital  Odense 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