{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T07:29:30Z","timestamp":1781162970602,"version":"3.54.1"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T00:00:00Z","timestamp":1757721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To evaluate the utility of CD48 as a marker to distinguish T\u2010lymphoblasts from mature T cells and NK cells in T\u2010lineage acute lymphoblastic leukemia (T\u2010ALL) for measurable residual disease (MRD) detection by flow cytometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>CD48 expression was assessed in T\u2010lymphoblasts and mature T cells from 51 diagnostic T\u2010ALL samples and 84 post\u2010therapy samples. Normalized median fluorescence intensity (nMFI) of CD48 was calculated as the ratio of MFI in the population of interest to that in mature B cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      At diagnosis, the median CD48 nMFI in T\u2010lymphoblasts was significantly lower (0.14) compared to mature T cells (1.29) (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001). In MRD\u2010positive post\u2010therapy samples, T\u2010lymphoblasts had a lower nMFI (0.22) than mature T cells (1.4), NK cells (1.06), and \u03b3\u03b4 T cells (1.09) (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>CD48 demonstrates consistent under\u2010expression in T\u2010lymphoblasts compared to mature T cells and NK cell cells, supporting its use as a reliable and informative marker for MRD detection in T\u2010ALL. Its inclusion would enhance the accuracy of flow cytometry\u2010based MRD panels.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ejh.70035","type":"journal-article","created":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T08:23:52Z","timestamp":1757751832000},"page":"591-598","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>CD48<\/scp>\n                    Is a Robust Marker and a Useful Addition for Assessment of Measurable Residual Disease in T\u2010Cell Acute Lymphoblastic Leukemia"],"prefix":"10.1111","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4235-0805","authenticated-orcid":false,"given":"Tharageswari","family":"Srinivasan","sequence":"first","affiliation":[{"name":"Department of Hematology Postgraduate Institute of Medical Education and Research  Chandigarh 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