{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T02:35:44Z","timestamp":1783218944459,"version":"3.54.6"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To determine the diagnostic value of disclosing monotypic T\u2010cell populations by expression analysis of the constant region 1 of T\u2010cell receptor \u03b2 (TRBC1) by flow cytometry, for the detection of T\u2010cell neoplasms, in a routine hematopathology practice setting.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A panel of antibodies ECD\u2010CD16\/PECy5.5\u2010CD4\/PECy7\u2010CD2\/APCAF700\u2010CD7\/KO\u2010CD8\/FITC\u2010TCR\u03b3\u03b4\/PE\u2010TRBC1\/APC\u2010CD26\/APC\u2010CD57\/APC\u2010CD30\/APCH7\u2010CD45\/BV421\u2010CD3 was applied. One thousand and twenty\u2010nine cases investigated during 26\u2009months were reviewed and categorized as monotypic or polytypic depending on the expression of TRBC1 in all identifiable CD3+ T\u2010cell subsets.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Although TRBC1 restriction proved to be sensitive (90.7%) with a high negative predictive value (99.1%), in some T\u2010cell neoplasms, particularly those with significant inflammation, including angioimmunoblastic T\u2010cell lymphoma, monotypic T\u2010cells were not detected. The specificity was high (95.6%) albeit with a modest positive predictive value (67.1%), reflecting T\u2010cell clones of uncertain significance (T\u2010CUS) without T\u2010cell neoplasm. Of these clones, 53.6% exhibited a T\u2010cell large granular lymphocytic leukemia\u2010like phenotype, most commonly found in the peripheral blood or bone marrow, while others were identified in association with unrelated primary tumors or other comorbidities. Seventy\u2010nine percent of the T\u2010CUS cases remained stable over time during 1\u201377\u2009months of follow\u2010up.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Including TRBC1 antibodies in a routine flow cytometry panel facilitates the identification of T\u2010cell neoplasms. The analysis must be interpreted within its clinical context since T\u2010cell lymphomas with a small and sometimes surface CD3\u2010negative neoplastic T cell population, may display normal patterns of TRBC1\u2010expression with a background of reactive T cells. Conversely, monotypic T\u2010cells can be found in the absence of T\u2010cell neoplasm.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ejh.70067","type":"journal-article","created":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T15:24:17Z","timestamp":1764429857000},"page":"245-255","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Restricted Expression of the Constant Region 1 of T\u2010Cell Receptor \u03b2 by Flow Cytometry Facilitates Detection of T\u2010Cell Neoplasms With High Specificity but Moderate Predictive Value"],"prefix":"10.1111","volume":"116","author":[{"given":"Nilofar","family":"Rajabian","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Department of Clinical Sciences, Division of Pathology Lund University  Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olof","family":"Axler","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Clinical Sciences, Division of Pathology Lund University  Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Wist\u00e9n","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Clinical Sciences, Division of Pathology Lund University  Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Porwit","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Clinical Sciences, Division of Pathology Lund University  Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mats","family":"Ehinger","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Clinical Sciences, Division of Pathology Lund University  Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,29]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2015.126300"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404481"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1309\/835B04QXGNNFNRJU"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1309\/3A32DTVMH640M2QA"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4444"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21881"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.14045"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tranon.2022.101552"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.22147"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13174379"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqy173"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21782"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-020-0568-2"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12368"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21233"},{"key":"e_1_2_13_17_1","first-page":"346","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Swerdlow S. 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