{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T16:13:49Z","timestamp":1777652029882,"version":"3.51.4"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100022542","name":"Breakthrough Cancer Research","doi-asserted-by":"publisher","award":["BCR\u20102021\u201001\u2010BPG"],"award-info":[{"award-number":["BCR\u20102021\u201001\u2010BPG"]}],"id":[{"id":"10.13039\/501100022542","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100022728","name":"Munster Technological University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100022728","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Following treatment, relapse of Multiple Myeloma (MM) occurs due to measurable residual disease (MRD). As therapeutic options expand, advances in response assessment become more critical, necessitating more sensitive MRD detection methods.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>This study aimed to improve the efficiency and sensitivity of a flow cytometry assay for MM MRD detection in blood and bone marrow. To achieve this, three pre\u2010enrichment methods were compared using 32 samples from patients with active MM and 122 samples from MM patients in remission. The methods compared were the Euroflow recommended erythrocyte bulk lysis (BL) method, density gradient medium (DGM) separation and negative selection (NS).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      By removing granulocytes, DGM facilitated the processing of a larger starting quantity of white blood cells (WBCs) (&gt;\u200960\u2009\u00d7\u200910\n                      <jats:sup>6<\/jats:sup>\n                      ) compared to BL (20\u2009\u00d7\u200910\n                      <jats:sup>6<\/jats:sup>\n                      ), achieving greater analytical sensitivity with a limit of detection of 5.2\u2009\u00d7\u200910\n                      <jats:sup>\u22127<\/jats:sup>\n                      vs. 2\u2009\u00d7\u200910\n                      <jats:sup>\u22126<\/jats:sup>\n                      . DGM separation also features a shorter processing time and is more cost\u2010effective. Although NS can also process large quantities of WBCs, the need for extra processing steps and substantially higher costs made this method the least suitable choice for clinical implementation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Pre\u2010enrichment via DGM separation can cost\u2010effectively reduce sample processing times and significantly increase the analytical sensitivity of MM MRD analysis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ejh.70076","type":"journal-article","created":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T02:22:28Z","timestamp":1764728548000},"page":"256-266","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Enhanced Detection of Multiple Myeloma Cells by Next\u2010Generation Flow Cytometry Following Density Gradient Medium Separation"],"prefix":"10.1111","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2923-2310","authenticated-orcid":false,"given":"Aisling","family":"O'Brien","sequence":"first","affiliation":[{"name":"Department of Biological Sciences Munster Technological University  Cork Ireland"},{"name":"Department of Immunology Cork University Hospital  Cork Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vitaliy","family":"Mykytiv","sequence":"additional","affiliation":[{"name":"Department of Haematology Cork University Hospital  Cork Ireland"},{"name":"Department of Medicine University College Cork  Cork Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fiona","family":"O'Halloran","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Munster Technological University  Cork Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,2]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(16)30206-6"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clml.2013.09.013"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-018-0063-7"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.237"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2016.205"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.120"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2014.98"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-05-1858"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.25709"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.13484"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.17118"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-08-3398"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/bcj.2016.117"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2017.166629"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2018.02.017"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.17713"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2019.112704"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21606"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/16078454.2021.1948208"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-019-0588-4"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2017.29"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41408-018-0153-9"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.13067"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21249"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beha.2010.09.002"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(95)00269-3"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-022-04771-5"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2025029234"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2023-189095"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-019-03833-5"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clml.2020.08.003"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.13927"},{"issue":"6","key":"e_1_2_11_34_1","first-page":"e190","article-title":"Characterisation and Relevance of CD138\u2010Negative Plasma Cells in Plasma Cell Myeloma","volume":"32","author":"Reid S.","year":"2010","journal-title":"International Journal of Laboratory Hematology"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.018689"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-06-510453"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.122"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21228"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clml.2019.09.622"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41408-023-00853-3"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14010087"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21398"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/pore.2024.1611882"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15072060"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.15003"}],"container-title":["European Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejh.70076","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ejh.70076","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejh.70076","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T02:07:54Z","timestamp":1770862074000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ejh.70076"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,2]]},"references-count":44,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/ejh.70076"],"URL":"https:\/\/doi.org\/10.1111\/ejh.70076","archive":["Portico"],"relation":{},"ISSN":["0902-4441","1600-0609"],"issn-type":[{"value":"0902-4441","type":"print"},{"value":"1600-0609","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,2]]},"assertion":[{"value":"2025-07-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}