{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T09:51:19Z","timestamp":1791366679933,"version":"4.3.1"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2026,11]]},"abstract":"ABSTRACT<\/jats:title>\n \n Objectives<\/jats:title>\n \n Myeloid\/lymphoid neoplasms with tyrosine kinase fusions are rare but important because many are highly sensitive to targeted therapy.\n PDGFRB<\/jats:italic>\n rearrangements are well\u2010established drivers, whereas\n TPM3<\/jats:italic>\n is an exceptionally rare fusion partner.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n We performed longitudinal analysis of an elderly patient with hypereosinophilia, integrating morphology, cytogenetics,\n PDGFRB<\/jats:italic>\n break\u2010apart FISH, whole\u2010transcriptome RNA sequencing, optical genome mapping, targeted myeloid DNA sequencing and exploratory fusion\u2010specific RT\u2010qPCR.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n A balanced t(1;5)(q21;q33) with\n PDGFRB<\/jats:italic>\n rearrangement in 70% of nuclei was identified at diagnosis. RNA sequencing detected an in\u2010frame\n TPM3<\/jats:italic>\n exon 8\u2010\n PDGFRB<\/jats:italic>\n exon 11 fusion, and optical genome mapping confirmed the balanced rearrangement. Low\u2010dose imatinib induced durable haematologic and cytogenetic remission of the fusion\u2010driven eosinophilic component. Serial DNA sequencing identified diagnostic\n ASXL1<\/jats:italic>\n ,\n EZH2<\/jats:italic>\n and low\u2010level\n SRSF2<\/jats:italic>\n abnormalities that persisted during remission, followed by acquisition or expansion of\n SETBP1<\/jats:italic>\n ,\n CSF3R<\/jats:italic>\n ,\n ETNK1<\/jats:italic>\n and a distinct\n SRSF2<\/jats:italic>\n variant during late neutrophilic progression, without re\u2010emergence of the\n PDGFRB<\/jats:italic>\n rearrangement.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n TPM3::PDGFRB<\/jats:italic>\n is a rare but actionable\n PDGFRB<\/jats:italic>\n fusion. Durable suppression of the fusion\u2010positive eosinophilic component was followed by a late CNL\u2010like neutrophilic phase. The clonal relationship between these phases remains unresolved. Integrated longitudinal fusion testing and myeloid mutational profiling are therefore essential to characterize disease complexity.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/ejh.70280","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T09:03:26Z","timestamp":1785834206000},"page":"1378-1390","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Longitudinal Molecular Characterization of a Rare\n \n TPM3::PDGFRB<\/i>\n <\/scp>\n \u2010Rearranged Myeloid\/Lymphoid Neoplasm With Late\n CNL<\/scp>\n \u2010Like Neutrophilic Evolution"],"prefix":"10.1111","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9423-9941","authenticated-orcid":false,"given":"Fr\u00e9d\u00e9ric","family":"Lambert","sequence":"first","affiliation":[{"name":"Center for Human Genetics University Hospital of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Koopmansch","sequence":"additional","affiliation":[{"name":"Center for Human Genetics University Hospital of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael Fernandez","family":"Carazo","sequence":"additional","affiliation":[{"name":"Center for Human Genetics University Hospital of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8121-5122","authenticated-orcid":false,"given":"Elisa","family":"Dardenne","sequence":"additional","affiliation":[{"name":"Center for Human Genetics University Hospital of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4311-6639","authenticated-orcid":false,"given":"Aurore","family":"Keutgens","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Hematology Laboratory University Hospital of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ga\u00ebtan","family":"Vanstraelen","sequence":"additional","affiliation":[{"name":"Department of Pathology University Hospital of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1711-2108","authenticated-orcid":false,"given":"Patrick","family":"Collins","sequence":"additional","affiliation":[{"name":"Department of Hematology Regional Hospital of East\u2010Belgium Verviers Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Menten","sequence":"additional","affiliation":[{"name":"Center for Human Genetics University Hospital of Li\u00e8ge Li\u00e8ge 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