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Recent<jats:italic>ex vivo<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>experiments have suggested that<jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>channel blockade increases the firing rate and\/or bursting in these neurons. The purpose of this study was therefore to characterize the source of<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>which activates the<jats:styled-content style=\"fixed-case\">mAHP<\/jats:styled-content>channels in serotonergic neurons. In voltage\u2010clamp experiments, an outward current was recorded at \u221260\u00a0mV after a depolarizing pulse to +100\u00a0mV. A supramaximal concentration of the SK channel blockers apamin or (\u2010)\u2010bicuculline methiodide blocked this outward current. This current was also sensitive to the broad<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>channel blocker<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>o<jats:sup>2+<\/jats:sup>and was partially blocked by both \u03c9\u2010conotoxin and mibefradil, which are blockers of<jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>\u2010type and<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010type<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>channels, respectively. Neither blockers of other voltage\u2010gated<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>channels nor<jats:styled-content style=\"fixed-case\">DBHQ<\/jats:styled-content>, an inhibitor of<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>\u2010induced<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>release, had any effect on the<jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>current. In current\u2010clamp experiments, m<jats:styled-content style=\"fixed-case\">AHP<\/jats:styled-content>s following action potentials were only blocked by \u03c9\u2010conotoxin and were unaffected by mibefradil. This was observed in slices from both juvenile and adult rats. Finally, when these neurons were induced to fire in an<jats:italic>in vivo<\/jats:italic>\u2010like pacemaker rate, only \u03c9\u2010conotoxin was able to increase their firing rate (by ~30%), an effect identical to the one previously reported for apamin. Our results demonstrate that<jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>\u2010type<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>channels are the only source of<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>which activates the<jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>channels underlying the m<jats:styled-content style=\"fixed-case\">AHP<\/jats:styled-content>. T\u2010type<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>channels may also activate<jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>channels under different circumstances.<\/jats:p>","DOI":"10.1111\/ejn.12408","type":"journal-article","created":{"date-parts":[[2013,11,5]],"date-time":"2013-11-05T02:24:25Z","timestamp":1383618265000},"page":"186-196","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Mechanism of the medium\u2010duration afterhyperpolarization in rat serotonergic neurons"],"prefix":"10.1111","volume":"39","author":[{"given":"Philippe","family":"Alix","sequence":"first","affiliation":[{"name":"Neurophysiology Unit GIGA Neurosciences University of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kumar","family":"Venkatesan","sequence":"additional","affiliation":[{"name":"Neurophysiology Unit GIGA Neurosciences University of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Scuv\u00e9e\u2010Moreau","sequence":"additional","affiliation":[{"name":"Neurophysiology Unit GIGA Neurosciences University of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Massotte","sequence":"additional","affiliation":[{"name":"Neurophysiology Unit GIGA Neurosciences University of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai\u2010Linh","family":"Nguyen Trung","sequence":"additional","affiliation":[{"name":"Neurophysiology Unit GIGA Neurosciences University of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte A.","family":"Cornil","sequence":"additional","affiliation":[{"name":"Behavioral Neuroendocrinology Unit GIGA Neurosciences University of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Seutin","sequence":"additional","affiliation":[{"name":"Neurophysiology Unit GIGA Neurosciences University of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,11,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-020911-153336"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"1786","DOI":"10.1523\/JNEUROSCI.02-12-01786.1982","article-title":"Intracellular identification of central noradrenergic and serotonergic neurons by a new double labeling procedure","volume":"2","author":"Aghajanian G.K.","year":"1982","journal-title":"J. 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