{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:17:22Z","timestamp":1791296242522,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,3,5]],"date-time":"2014-03-05T00:00:00Z","timestamp":1393977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Microglia colonise the brain parenchyma at early stages of development and accumulate in specific regions where they participate in cell death, angiogenesis, neurogenesis and synapse elimination. A recurring feature of embryonic microglial is their association with developing axon tracts, which, together with <jats:italic>in vitro<\/jats:italic> data, supports the idea of a physiological role for microglia in neurite development. Yet the demonstration of this role of microglia is lacking. Here, we have studied the consequences of microglial dysfunction on the formation of the corpus callosum, the largest commissure of the mammalian brain, which shows consistent microglial accumulation during development. We studied two models of microglial dysfunction: the loss\u2010of\u2010function of <jats:styled-content style=\"fixed-case\">DAP<\/jats:styled-content>12, a key microglial\u2010specific signalling molecule, and a model of maternal inflammation by peritoneal injection of lipopolysaccharide at embryonic day (E)15.5. We also took advantage of the <jats:italic>Pu.1<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> mouse line, which is devoid of microglia. We performed transcriptional profiling of maternally inflamed and <jats:italic>Dap12<\/jats:italic>\u2010mutant microglia at E17.5. The two treatments principally down\u2010regulated genes involved in nervous system development and function, particularly in neurite formation. We then analysed the developmental consequences of these microglial dysfunctions on the formation of the corpus callosum. We show that all three models of altered microglial activity resulted in the defasciculation of dorsal callosal axons. Our study demonstrates that microglia display a neurite\u2010development\u2010promoting function and are genuine actors of corpus callosum development. It further shows that microglial activation impinges on this function, thereby revealing that prenatal inflammation impairs neuronal development through a loss of trophic support.<\/jats:p>","DOI":"10.1111\/ejn.12508","type":"journal-article","created":{"date-parts":[[2014,3,5]],"date-time":"2014-03-05T08:42:44Z","timestamp":1394008964000},"page":"1551-1557","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":188,"title":["Microglia shape corpus callosum axon tract fasciculation: functional impact of prenatal inflammation"],"prefix":"10.1111","volume":"39","author":[{"given":"Lorena","family":"Pont\u2010Lezica","sequence":"first","affiliation":[{"name":"Institut de Biologie de l'Ecole Normale Sup\u00e9rieure F\u201075005 Paris France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale Paris France"},{"name":"Centre National de la Recherche Scientifique Unit\u00e9 Mixte de Recherche Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wouter","family":"Beumer","sequence":"additional","affiliation":[{"name":"Department of Immunology Erasmus MC Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Colasse","sequence":"additional","affiliation":[{"name":"Institut de Biologie de l'Ecole Normale Sup\u00e9rieure F\u201075005 Paris France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale Paris France"},{"name":"Centre National de la Recherche Scientifique Unit\u00e9 Mixte de Recherche Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hemmo","family":"Drexhage","sequence":"additional","affiliation":[{"name":"Department of Immunology Erasmus MC Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjan","family":"Versnel","sequence":"additional","affiliation":[{"name":"Department of Immunology Erasmus MC Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Bessis","sequence":"additional","affiliation":[{"name":"Institut de Biologie de l'Ecole Normale Sup\u00e9rieure F\u201075005 Paris France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale Paris France"},{"name":"Centre National de la Recherche Scientifique Unit\u00e9 Mixte de Recherche Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,3,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-11-4089"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0212100"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2010.03.005"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490380213"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903300110"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3441-12.2013"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(90)90085-T"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.22480"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.11.031"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.09.002"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2010.10.002"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1194637"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1204"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2001.0393"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(83)90201-8"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.11.4106-4114.2000"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.20562"},{"key":"e_1_2_6_20_1","first-page":"29","article-title":"Some aspects of amoeboid microglia in the corpus callosum and neighbouring regions of neonatal rats","volume":"121","author":"Ling E.A.","year":"1976","journal-title":"J. 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