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To examine whether the plateau potentials are accompanied by an elevation of intracellular<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>and to determine the source of<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>elevation, we performed<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>imaging during the plateau potential. Neurons were loaded with<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>indicator fluo\u20104, and the plateau potentials were generated either synaptically in the presence of glutamate transporter antagonist or by iontophoretically applying<jats:styled-content style=\"fixed-case\">NMDA<\/jats:styled-content>. We have found that a transient elevation in intracellular<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>accompanies the plateau potential. The synaptically induced plateau potential and the<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>elevation were blocked by 5,7\u2010dichlorokynurenic acid (5,7\u2010d<jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>), an antagonist for the glycine\u2010binding sites of<jats:styled-content style=\"fixed-case\">NMDAR<\/jats:styled-content>. A mixture of<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>d<jats:sup>2+<\/jats:sup>and tetrodotoxin did not block<jats:styled-content style=\"fixed-case\">NMDA<\/jats:styled-content>\u2010induced plateau potentials, but completely abolished the accompanying<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>elevation in both the presence and absence of<jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>g<jats:sup>2+<\/jats:sup>ions in the bathing solution. The<jats:styled-content style=\"fixed-case\">NMDA<\/jats:styled-content>\u2010induced plateau potential was blocked by further adding 5,7\u2010d<jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>. Our results show that the<jats:styled-content style=\"fixed-case\">NMDAR<\/jats:styled-content>\u2010mediated plateau potential is accompanied by elevation of intracellular<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>that is primarily caused by the influx of<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>through voltage\u2010gated<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>channels.<\/jats:p>","DOI":"10.1111\/ejn.12555","type":"journal-article","created":{"date-parts":[[2014,3,27]],"date-time":"2014-03-27T12:24:40Z","timestamp":1395923080000},"page":"1613-1623","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Intracellular calcium elevation during plateau potentials mediated by extrasynaptic<scp>NMDA<\/scp>receptor activation in rat hippocampal<scp>CA<\/scp>1 pyramidal neurons is primarily due to calcium entry through voltage\u2010gated calcium channels"],"prefix":"10.1111","volume":"39","author":[{"given":"Yoshiaki","family":"Oda","sequence":"first","affiliation":[{"name":"Laboratory of Cellular Neurobiology School of Life Sciences Tokyo University of Pharmacy and Life Sciences Hachioji Tokyo 192\u20100392 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Kodama","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Neurobiology School of Life Sciences Tokyo University of Pharmacy and Life Sciences Hachioji Tokyo 192\u20100392 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadahiro","family":"Tsuchiya","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Neurobiology School of Life Sciences Tokyo University of Pharmacy and Life Sciences Hachioji Tokyo 192\u20100392 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2728-0060","authenticated-orcid":false,"given":"Masashi","family":"Inoue","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Neurobiology School of Life Sciences Tokyo University of Pharmacy and Life Sciences Hachioji Tokyo 192\u20100392 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyoshi","family":"Miyakawa","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Neurobiology School of Life Sciences Tokyo University of Pharmacy and Life Sciences Hachioji Tokyo 192\u20100392 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,3,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2010.03.003"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.22444"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2006.12.017"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000190"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-01-00047.1995"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(96)00880-1"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-09-05471.1994"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp020738"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00586-X"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.57.4.5"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2777-11.2011"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1995.73.6.2553"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/stke.2552004re16"},{"key":"e_1_2_6_15_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/S0031-6997(24)01394-2","article-title":"The glutamate receptor ion channels","volume":"51","author":"Dingledine D.","year":"1999","journal-title":"Pharmacol. 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