{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:30:26Z","timestamp":1788870626605,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,7,21]],"date-time":"2014-07-21T00:00:00Z","timestamp":1405900800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","award":["31003A_138246\/1"],"award-info":[{"award-number":["31003A_138246\/1"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004191","name":"Novo Nordisk","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004191","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2014,10]]},"abstract":"Abstract<\/jats:title>\n \n Amylin reduces meal size by activating noradrenergic neurons in the area postrema (\n AP<\/jats:styled-content>\n ). Neurons in the\n AP<\/jats:styled-content>\n also mediate the eating\u2010inhibitory effects of salmon calcitonin (\n sCT<\/jats:styled-content>\n ), a potent amylin agonist, but the phenotypes of the neurons mediating its effect are unknown. Here we investigated whether\n sCT<\/jats:styled-content>\n activates similar neuronal populations to amylin, and if its anorectic properties also depend on\n AP<\/jats:styled-content>\n function. Male rats underwent\n AP<\/jats:styled-content>\n lesion (\n APX<\/jats:styled-content>\n ) or sham surgery. Meal patterns were analysed under\n ad libitum<\/jats:italic>\n and post\u2010deprivation conditions. The importance of the\n AP<\/jats:styled-content>\n in mediating the anorectic action of\n sCT<\/jats:styled-content>\n was examined in feeding experiments of dose\u2013response effects of\n sCT<\/jats:styled-content>\n in\n APX<\/jats:styled-content>\n vs. sham rats. The effect of\n sCT<\/jats:styled-content>\n to induce Fos expression was compared between surgery groups, and relative to amylin. The phenotype of Fos\u2010expressing neurons in the brainstem was examined by testing for the co\u2010expression of dopamine beta hydroxylase (\n DBH<\/jats:styled-content>\n ) or tryptophan hydroxylase (\n TPH<\/jats:styled-content>\n ). By measuring the apposition of vesicular glutamate transporter\u20102 (\n VGLUT<\/jats:styled-content>\n 2)\u2010positive boutons, potential glutamatergic input to amylin\u2010 and\n sCT<\/jats:styled-content>\n \u2010activated\n AP<\/jats:styled-content>\n neurons was compared. Similar to amylin, an intact\n AP<\/jats:styled-content>\n was necessary for\n sCT<\/jats:styled-content>\n to reduce eating. Further, co\u2010expression between Fos activation and\n DBH<\/jats:styled-content>\n after amylin or\n sCT<\/jats:styled-content>\n did not differ markedly, while co\u2010localization of Fos and\n TPH<\/jats:styled-content>\n was minor. Approximately 95% of neurons expressing Fos and\n DBH<\/jats:styled-content>\n after amylin or\n sCT<\/jats:styled-content>\n treatment were closely apposed to\n VGLUT<\/jats:styled-content>\n 2\u2010positive boutons. Our study suggests that the hindbrain pathways engaged by amylin and\n sCT<\/jats:styled-content>\n share many similarities, including the mediation by\n AP<\/jats:styled-content>\n neurons.\n <\/jats:p>","DOI":"10.1111\/ejn.12672","type":"journal-article","created":{"date-parts":[[2014,7,21]],"date-time":"2014-07-21T01:39:27Z","timestamp":1405906767000},"page":"3055-3066","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["The role of the area postrema in the anorectic effects of amylin and salmon calcitonin: behavioral and neuronal phenotyping"],"prefix":"10.1111","volume":"40","author":[{"given":"Fiona E.","family":"Braegger","sequence":"first","affiliation":[{"name":"Institute of Veterinary Physiology University of Zurich Zurich 8057 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lori","family":"Asarian","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Physiology University of Zurich Zurich 8057 Switzerland"},{"name":"Zurich Center of Integrative Human Physiology Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Dahl","sequence":"additional","affiliation":[{"name":"NovoNordisk A\/S Maaloev Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas A.","family":"Lutz","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Physiology University of Zurich Zurich 8057 Switzerland"},{"name":"Zurich Center of Integrative Human Physiology Zurich Switzerland"},{"name":"Institute of Laboratory Animal Science University of Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina N.","family":"Boyle","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Physiology University of Zurich Zurich 8057 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,7,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1550-8528.1995.tb00493.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2003.10.040"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2011.08.005"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(02)02552-9"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.39.6.752"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.56.1.235"},{"key":"e_1_2_6_8_1","first-page":"26","article-title":"Area postrem (AP)\u2010lesions block the regulation of gastric emptying by amylin","volume":"10","author":"Edwards G.L.","year":"1998","journal-title":"Neurogastroent. 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