{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T11:21:04Z","timestamp":1772882464001,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,7,16]],"date-time":"2014-07-16T00:00:00Z","timestamp":1405468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R21NS078517"],"award-info":[{"award-number":["R21NS078517"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human umbilical cord blood (<jats:styled-content style=\"fixed-case\">HUCB<\/jats:styled-content>) cells have shown efficacy in rodent models of focal ischemia and <jats:italic>in vitro<\/jats:italic> systems that recapitulate stroke conditions. One potential mechanism of protection is through secretion of soluble factors that protect neurons and oligodendrocytes (<jats:styled-content style=\"fixed-case\">OL<\/jats:styled-content>s) from oxidative stress. To overcome practical issues with cellular therapies, identification of soluble factors released by <jats:styled-content style=\"fixed-case\">HUCB<\/jats:styled-content> and other stem cells may pave the way for treatment modalities that are safer for a larger percentage of stroke patients. Among these soluble factors is leukemia inhibitory factor (<jats:styled-content style=\"fixed-case\">LIF<\/jats:styled-content>), a cytokine that exerts pleiotropic effects on cell survival. Here, data show that <jats:styled-content style=\"fixed-case\">LIF<\/jats:styled-content> effectively reduced infarct volume, reduced white matter injury and improved functional outcomes when administered to rats following permanent middle cerebral artery occlusion. To further explore downstream signaling, primary oligodendrocyte cultures were exposed to oxygen\u2013glucose deprivation to mimic stroke conditions. <jats:styled-content style=\"fixed-case\">LIF<\/jats:styled-content> significantly reduced lactate dehydrogenase release from <jats:styled-content style=\"fixed-case\">OL<\/jats:styled-content>s, reduced superoxide dismutase activity and induced peroxiredoxin 4 (<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>rdx4) transcript. Additionally, the protective and antioxidant capacity of <jats:styled-content style=\"fixed-case\">LIF<\/jats:styled-content> was negated by both <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>kt inhibition and co\u2010incubation with <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>rdx4\u2010neutralising antibodies, establishing a role for the <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>kt signaling pathway and <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>rdx4\u2010mediated antioxidation in <jats:styled-content style=\"fixed-case\">LIF<\/jats:styled-content> protection.<\/jats:p>","DOI":"10.1111\/ejn.12675","type":"journal-article","created":{"date-parts":[[2014,7,16]],"date-time":"2014-07-16T12:35:06Z","timestamp":1405514106000},"page":"3111-3119","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Leukemia inhibitor factor promotes functional recovery and oligodendrocyte survival in rat models of focal ischemia"],"prefix":"10.1111","volume":"40","author":[{"given":"Derrick D.","family":"Rowe","sequence":"first","affiliation":[{"name":"Department of Molecular Pharmacology and Physiology Morsani College of Medicine University of South Florida 12901 Bruce B. Downs Blvd. Tampa FL 33612 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa A.","family":"Collier","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Physiology Morsani College of Medicine University of South Florida 12901 Bruce B. Downs Blvd. Tampa FL 33612 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hilary A.","family":"Seifert","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Physiology Morsani College of Medicine University of South Florida 12901 Bruce B. Downs Blvd. Tampa FL 33612 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cortney B.","family":"Chapman","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Physiology Morsani College of Medicine University of South Florida 12901 Bruce B. Downs Blvd. Tampa FL 33612 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher C.","family":"Leonardo","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Physiology Morsani College of Medicine University of South Florida 12901 Bruce B. Downs Blvd. Tampa FL 33612 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alison E.","family":"Willing","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Physiology Morsani College of Medicine University of South Florida 12901 Bruce B. Downs Blvd. Tampa FL 33612 USA"},{"name":"Center of Excellence for Aging and Brain Repair Morsani College of Medicine University of South Florida Tampa FL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keith R.","family":"Pennypacker","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Physiology Morsani College of Medicine University of South Florida 12901 Bruce B. Downs Blvd. Tampa FL 33612 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,7,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/156720206776875849"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747-4949.2009.00387.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12233"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.jnen.0000235855.77716.25"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clp.2013.11.001"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.118.1.283"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)67521-0"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0602-613"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20321"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1136(199606)17:2<83::AID-GLIA1>3.0.CO;2-7"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2005.02.021"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490420307"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.21857"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2009.08.051"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2008.07.015"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2002.040"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022401315721"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.05.002"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0149-7634(96)00005-X"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1136(199804)22:4<371::AID-GLIA6>3.0.CO;2-6"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2005.10.006"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20177"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1681.2000.03289.x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3597"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199805000-00007"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.120.1.143"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.85.3.890"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pediatrneurol.2003.10.001"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2008.05570.x"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.21-1-5"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.15.5948"},{"key":"e_1_2_7_33_1","first-page":"847","article-title":"A myelosclerotic syndrome in mice engrafted with cells producing high levels of leukemia inhibitory factor (LIF)","volume":"3","author":"Metcalf D.","year":"1989","journal-title":"Leukemia"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(95)00063-J"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2007.03.019"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.16.9703"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2010.09.078"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.296434"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(96)01387-X"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11481-012-9406-8"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1515\/jbcpp-2013-0121"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700641"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-1331.2007.01995.x"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8272873"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7871433"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jcbfm.9600061"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000141681.06735.9b"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2005.14.595"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2005.05.003"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1385\/MN:34:3:249"}],"container-title":["European Journal of Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fejn.12675","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejn.12675","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T20:11:45Z","timestamp":1696363905000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ejn.12675"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7,16]]},"references-count":49,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["10.1111\/ejn.12675"],"URL":"https:\/\/doi.org\/10.1111\/ejn.12675","archive":["Portico"],"relation":{},"ISSN":["0953-816X","1460-9568"],"issn-type":[{"value":"0953-816X","type":"print"},{"value":"1460-9568","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,16]]},"assertion":[{"value":"2014-03-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-06-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-07-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}