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We provided evidence for a central role of the N\u2010type Cav channel subunit Cav2.2 in presynaptic transmitter release at these synapses. Striking Cav2.2 immunoreactivity was localised to the glomerular neuropil of the main olfactory bulb (<jats:styled-content style=\"fixed-case\">MOB<\/jats:styled-content>) and accessory olfactory bulb (<jats:styled-content style=\"fixed-case\">AOB<\/jats:styled-content>), and co\u2010localised with presynaptic molecules such as bassoon. Voltage\u2010clamp recordings of sensory nerve\u2010evoked, excitatory postsynaptic currents (<jats:styled-content style=\"fixed-case\">EPSC<\/jats:styled-content>s) in mitral\/tufted (M\/T) and superficial tufted cells of the <jats:styled-content style=\"fixed-case\">MOB<\/jats:styled-content> and mitral cells of the <jats:styled-content style=\"fixed-case\">AOB<\/jats:styled-content>, in combination with established subtype\u2010specific Cav channel toxins, indicated a predominant role of N\u2010type channels in transmitter release at these synapses, whereas L\u2010type, P\/Q\u2010type, and R\u2010type channels had either no or only relatively minor contributions. In <jats:italic>Cacna1b<\/jats:italic> mutant mice lacking the Cav2.2 (\u03b11B) subunit of N\u2010type channels, olfactory nerve\u2010evoked M\/T cell <jats:styled-content style=\"fixed-case\">EPSC<\/jats:styled-content>s were not reduced but became blocker\u2010resistant, thus indicating a major reorganisation and compensation of Cav channel subunits as a result of the Cav2.2 deletion at this synapse. Cav2.2\u2010deficient mice also revealed that Cav2.2 was critically required for paired\u2010pulse depression of olfactory nerve\u2010evoked <jats:styled-content style=\"fixed-case\">EPSC<\/jats:styled-content>s in M\/T cells of the <jats:styled-content style=\"fixed-case\">MOB<\/jats:styled-content>, and they demonstrated an essential requirement for Cav2.2 in vomeronasal nerve\u2010evoked <jats:styled-content style=\"fixed-case\">EPSC<\/jats:styled-content>s of <jats:styled-content style=\"fixed-case\">AOB<\/jats:styled-content> mitral cells. Thus, <jats:italic>Cacna1b<\/jats:italic> loss\u2010of\u2010function mutations are unlikely to cause general anosmia but <jats:italic>Cacna1b<\/jats:italic> emerges as a strong candidate in the search for mutations causing altered olfactory perception, such as changes in general olfactory sensitivity and altered social responses to chemostimuli.<\/jats:p>","DOI":"10.1111\/ejn.12713","type":"journal-article","created":{"date-parts":[[2014,9,5]],"date-time":"2014-09-05T08:15:23Z","timestamp":1409904923000},"page":"3422-3435","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Altered synaptic transmission at olfactory and vomeronasal nerve terminals in mice lacking N\u2010type calcium channel Cav2.2"],"prefix":"10.1111","volume":"40","author":[{"given":"Jan","family":"Weiss","sequence":"first","affiliation":[{"name":"Department of Physiology University of Saarland School of Medicine Kirrbergerstrasse Building 58 D\u201066421 Homburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Pyrski","sequence":"additional","affiliation":[{"name":"Department of Physiology University of Saarland School of Medicine Kirrbergerstrasse Building 58 D\u201066421 Homburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"Weissgerber","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology University of Saarland School of Medicine Homburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Zufall","sequence":"additional","affiliation":[{"name":"Department of Physiology University of Saarland School of Medicine Kirrbergerstrasse Building 58 D\u201066421 Homburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,9,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.03-01-00069.1983"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05404"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1249168"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-05-02011.2000"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.57.4.4"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.57.4.5"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1107770108"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2012.04.011"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1253136"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2005.04.030"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.187807"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.11.021"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5317-08.2009"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.142.2.499"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00430-6"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.39.073003.093937"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/chemse\/bjl032"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2007.05400.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5580-11.2012"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-17-06904.2003"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2374-05.2005"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.081089398"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)81013-2"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1515\/revneuro-2011-0070"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/1744-8069-9-58"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0004.2011.01776.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"crossref","first-page":"6232","DOI":"10.1016\/S0021-9258(20)81849-3","article-title":"Isolation and characterization of rat olfactory marker protein","volume":"251","author":"Keller A.","year":"1976","journal-title":"J. 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