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The exact role of LRIT<\/jats:styled-content>3 in ON<\/jats:styled-content>\u2010bipolar cell signaling cascade remains to be elucidated. Recently, we have characterized a novel mouse model lacking Lrit3<\/jats:italic> [no b\u2010wave 6,<\/jats:italic> (Lrit3<\/jats:italic>nob6<\/jats:italic>\/nob6<\/jats:italic><\/jats:sup>)], which displays similar abnormalities to patients with cCSNB<\/jats:styled-content> with LRIT<\/jats:styled-content>3<\/jats:italic> mutations. Here we compare the localization of components of the ON<\/jats:styled-content>\u2010bipolar cell signaling cascade in wild\u2010type and Lrit3<\/jats:italic>nob6<\/jats:italic>\/nob6<\/jats:italic><\/jats:sup> retinal sections by immunofluorescence confocal microscopy. An anti\u2010LRIT<\/jats:styled-content>3 antibody was generated. Immunofluorescent staining of LRIT<\/jats:styled-content>3 in wild\u2010type mice revealed a specific punctate labeling in the outer plexiform layer (OPL<\/jats:styled-content>), which was absent in Lrit3<\/jats:italic>nob6<\/jats:italic>\/nob6<\/jats:italic><\/jats:sup> mice. LRIT<\/jats:styled-content>3 did not co\u2010localize with ribeye or calbindin but co\u2010localized with mG<\/jats:styled-content>luR6. TRPM<\/jats:styled-content>1 staining was severely decreased at the dendritic tips of all depolarizing bipolar cells in Lrit3<\/jats:italic>nob6<\/jats:italic>\/nob6<\/jats:italic><\/jats:sup> mice. mG<\/jats:styled-content>luR6, GPR<\/jats:styled-content>179, RGS<\/jats:styled-content>7, RGS<\/jats:styled-content>11 and G\u03b25 immunofluorescence was absent at the dendritic tips of cone ON<\/jats:styled-content>\u2010bipolar cells in Lrit3<\/jats:italic>nob6<\/jats:italic>\/nob6<\/jats:italic><\/jats:sup> mice, while it was present at the dendritic tips of rod bipolar cells. Furthermore, peanut agglutinin (PNA<\/jats:styled-content>) labeling was severely reduced in the OPL<\/jats:styled-content> in Lrit3<\/jats:italic>nob6<\/jats:italic>\/nob6<\/jats:italic><\/jats:sup> mice. This study confirmed the localization of LRIT<\/jats:styled-content>3 at the dendritic tips of depolarizing bipolar cells in mouse retina and demonstrated the dependence of TRPM<\/jats:styled-content>1 localization on the presence of LRIT<\/jats:styled-content>3. As tested components of the ON<\/jats:styled-content>\u2010bipolar cell signaling cascade and PNA<\/jats:styled-content> revealed disrupted localization, an additional function of LRIT<\/jats:styled-content>3 in cone synapse formation is suggested. These results point to a possibly different regulation of the mG<\/jats:styled-content>luR6 signaling cascade between rod and cone ON<\/jats:styled-content>\u2010bipolar cells.<\/jats:p>","DOI":"10.1111\/ejn.12959","type":"journal-article","created":{"date-parts":[[2015,5,22]],"date-time":"2015-05-22T07:02:05Z","timestamp":1432278125000},"page":"1966-1975","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":65,"title":["LRIT<\/scp>3 is essential to localize TRPM<\/scp>1 to the dendritic tips of depolarizing bipolar cells and may play a role in cone synapse formation"],"prefix":"10.1111","volume":"42","author":[{"given":"Marion","family":"Neuill\u00e9","sequence":"first","affiliation":[{"name":"INSERM U968 Paris F\u201075012 France"},{"name":"CNRS UMR_7210 Paris F\u201075012 France"},{"name":"Sorbonne Universit\u00e9s UPMC Univ Paris 06 UMR_S 968 Institut de la Vision Paris F\u201075012 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine W.","family":"Morgans","sequence":"additional","affiliation":[{"name":"Department of Physiology & Pharmacology Oregon Health & Science University Portland OR 97239 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Neuroscience The Scripps Research Institute Jupiter FL 33458 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elise","family":"Orhan","sequence":"additional","affiliation":[{"name":"INSERM U968 Paris F\u201075012 France"},{"name":"CNRS UMR_7210 Paris F\u201075012 France"},{"name":"Sorbonne Universit\u00e9s UPMC Univ Paris 06 UMR_S 968 Institut de la Vision Paris F\u201075012 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Michiels","sequence":"additional","affiliation":[{"name":"INSERM U968 Paris F\u201075012 France"},{"name":"CNRS UMR_7210 Paris F\u201075012 France"},{"name":"Sorbonne Universit\u00e9s UPMC Univ Paris 06 UMR_S 968 Institut de la Vision Paris F\u201075012 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9\u2010Alain","family":"Sahel","sequence":"additional","affiliation":[{"name":"INSERM U968 Paris F\u201075012 France"},{"name":"CNRS UMR_7210 Paris F\u201075012 France"},{"name":"Sorbonne Universit\u00e9s UPMC Univ Paris 06 UMR_S 968 Institut de la Vision Paris F\u201075012 France"},{"name":"Centre Hospitalier National d'Ophtalmologie des Quinze\u2010Vingts DHU ViewMaintain INSERM\u2010DHOS CIC 1423 Paris F\u201075012 France"},{"name":"Institute of Ophthalmology University College of London London EC1V 9EL UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Audo","sequence":"additional","affiliation":[{"name":"INSERM U968 Paris F\u201075012 France"},{"name":"CNRS UMR_7210 Paris F\u201075012 France"},{"name":"Sorbonne Universit\u00e9s UPMC Univ Paris 06 UMR_S 968 Institut de la Vision Paris F\u201075012 France"},{"name":"Centre Hospitalier National d'Ophtalmologie des Quinze\u2010Vingts DHU ViewMaintain INSERM\u2010DHOS CIC 1423 Paris F\u201075012 France"},{"name":"Institute of Ophthalmology University College of London London EC1V 9EL UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M.","family":"Duvoisin","sequence":"additional","affiliation":[{"name":"Department of Physiology & Pharmacology Oregon Health & Science University Portland OR 97239 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirill A.","family":"Martemyanov","sequence":"additional","affiliation":[{"name":"Department of Neuroscience The Scripps Research Institute Jupiter FL 33458 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Zeitz","sequence":"additional","affiliation":[{"name":"INSERM U968 Paris F\u201075012 France"},{"name":"CNRS UMR_7210 Paris F\u201075012 France"},{"name":"Sorbonne Universit\u00e9s UPMC Univ Paris 06 UMR_S 968 Institut de la Vision Paris F\u201075012 France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2006.05053.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902210103"},{"key":"e_1_2_6_4_1","first-page":"546","article-title":"Specific binding of peanut lectin to a class of retinal photoreceptor cells. 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