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Long non\u2010coding <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) are <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> transcripts &gt;\u00a0200 nucleotides in length without protein\u2010coding potential. They regulate gene expression at epigenetic, transcriptional and post\u2010transcriptional levels, and are thus involved in many biological processes and human diseases. However, the role and mechanisms of lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in regulating the responses of <jats:styled-content style=\"fixed-case\">DRG<\/jats:styled-content> neurons to sciatic nerve injury are not fully investigated. We have previously analysed the expression profiles of lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s in L4\u20106 <jats:styled-content style=\"fixed-case\">DRG<\/jats:styled-content>s, following rat sciatic nerve transection, by microarray analysis, and constructed a coexpression network of dysregulated lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s and coding genes. In this study, one of these dysregulated lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s, uc.217, was chosen for detailed examination of its expression changes and regulative functions in regenerative <jats:styled-content style=\"fixed-case\">DRG<\/jats:styled-content> neuronal outgrowth. Quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and <jats:italic>in\u00a0situ<\/jats:italic> hybridisation confirmed that the expression of uc.217 was down\u2010regulated in <jats:styled-content style=\"fixed-case\">DRG<\/jats:styled-content> neurons after sciatic nerve injury. Silencing of uc.217 expression by small interfering <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> could significantly promote neurite outgrowth in cultured <jats:styled-content style=\"fixed-case\">DRG<\/jats:styled-content> neurons. Moreover, bioinformatic analysis and experimental validation were performed to identify several potential targets of uc.217, which were involved in the regulation of <jats:styled-content style=\"fixed-case\">DRG<\/jats:styled-content> neuron outgrowth. Collectively, our results suggested that a new lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>, uc.217, played an important regulative role in peripheral nerve regeneration.<\/jats:p>","DOI":"10.1111\/ejn.12966","type":"journal-article","created":{"date-parts":[[2015,5,28]],"date-time":"2015-05-28T14:06:21Z","timestamp":1432821981000},"page":"1718-1725","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Long non\u2010coding <scp>RNA<\/scp> uc.217 regulates neurite outgrowth in dorsal root ganglion neurons following peripheral nerve injury"],"prefix":"10.1111","volume":"42","author":[{"given":"Chun","family":"Yao","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Neuroregeneration Co\u2010innovation Center of Neuroregeneration Nantong University Nantong 226001 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Neuroregeneration Co\u2010innovation Center of Neuroregeneration Nantong University Nantong 226001 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honghong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Neuroregeneration Co\u2010innovation Center of Neuroregeneration Nantong University Nantong 226001 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songlin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Neuroregeneration Co\u2010innovation Center of Neuroregeneration Nantong University Nantong 226001 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianmei","family":"Qian","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Neuroregeneration Co\u2010innovation Center of Neuroregeneration Nantong University Nantong 226001 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Ding","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Neuroregeneration Co\u2010innovation Center of Neuroregeneration Nantong University Nantong 226001 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaosong","family":"Gu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Neuroregeneration Co\u2010innovation Center of Neuroregeneration Nantong University Nantong 226001 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Yu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Neuroregeneration Co\u2010innovation Center of Neuroregeneration Nantong University Nantong 226001 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,6,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039674"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.6271-09.2010"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3679"},{"key":"e_1_2_7_5_1","first-page":"a001867","article-title":"Guidance molecules in axon regeneration","volume":"2","author":"Giger R.J.","year":"2010","journal-title":"Cold Sh. 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