{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:22:25Z","timestamp":1760217745289,"version":"3.37.3"},"reference-count":60,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,2,11]],"date-time":"2016-02-11T00:00:00Z","timestamp":1455148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002341","name":"Suomen Akatemia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007247","name":"Svenska Kulturfonden","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007247","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004155","name":"Magnus Ehrnroothin S\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004155","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004913","name":"Universit\u00e0 degli Studi di Palermo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004913","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Peroxisome proliferator\u2010activated receptor gamma coactivator\u20101\u03b1 (<jats:styled-content style=\"fixed-case\">PGC<\/jats:styled-content>\u20101\u03b1) is a transcriptional coactivator involved in the regulation of mitochondrial biogenesis and cell defense. The functions of <jats:styled-content style=\"fixed-case\">PGC<\/jats:styled-content>\u20101\u03b1 in physiology of brain mitochondria are, however, not fully understood. To address this we have studied wild\u2010type and transgenic mice with a two\u2010fold overexpression of <jats:styled-content style=\"fixed-case\">PGC<\/jats:styled-content>\u20101\u03b1 in brain neurons. Data showed that the relative number and basal respiration of brain mitochondria were increased in <jats:styled-content style=\"fixed-case\">PGC<\/jats:styled-content>\u20101\u03b1 transgenic mice compared with wild\u2010type mitochondria. These changes occurred concomitantly with altered levels of proteins involved in oxidative phosphorylation (<jats:styled-content style=\"fixed-case\">OXPHOS<\/jats:styled-content>) as studied by proteomic analyses and immunoblottings. Cultured hippocampal neurons from <jats:styled-content style=\"fixed-case\">PGC<\/jats:styled-content>\u20101\u03b1 transgenic mice were more resistant to cell degeneration induced by the glutamate receptor agonist kainic acid. <jats:italic>In vivo<\/jats:italic> kainic acid induced excitotoxic cell death in the hippocampus at 48\u00a0h in wild\u2010type mice but significantly less so in <jats:styled-content style=\"fixed-case\">PGC<\/jats:styled-content>\u20101\u03b1 transgenic mice. However, at later time points cell degeneration was also evident in the transgenic mouse hippocampus, indicating that <jats:styled-content style=\"fixed-case\">PGC<\/jats:styled-content>\u20101\u03b1 overexpression can induce a delay in cell death. Immunoblotting showed that X\u2010linked inhibitor of apoptosis protein (<jats:styled-content style=\"fixed-case\">XIAP<\/jats:styled-content>) was increased in <jats:styled-content style=\"fixed-case\">PGC<\/jats:styled-content>\u20101\u03b1 transgenic hippocampus with no significant changes in Bcl\u20102 or Bcl\u2010X. Collectively, these results show that <jats:styled-content style=\"fixed-case\">PGC<\/jats:styled-content>\u20101\u03b1 overexpression contributes to enhanced neuronal viability by stimulating mitochondria number and respiration and increasing levels of <jats:styled-content style=\"fixed-case\">OXPHOS<\/jats:styled-content> proteins and the anti\u2010apoptotic protein <jats:styled-content style=\"fixed-case\">XIAP<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/ejn.13157","type":"journal-article","created":{"date-parts":[[2016,1,7]],"date-time":"2016-01-07T13:32:59Z","timestamp":1452173579000},"page":"626-639","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Peroxisome proliferator\u2010activated receptor\u2010\u03b3 coactivator\u20101\u03b1 mediates neuroprotection against excitotoxic brain injury in transgenic mice: role of mitochondria and X\u2010linked inhibitor of apoptosis protein"],"prefix":"10.1111","volume":"43","author":[{"given":"Johanna","family":"M\u00e4kel\u00e4","sequence":"first","affiliation":[{"name":"Medicum Department of Biochemistry and Developmental Biology Medical Faculty University of Helsinki POB 63, 00014, Haartmaninkatu 8 FIN\u201000290 Helsinki Finland"},{"name":"Minerva Medical Research Institute Biomedicum\u20102 Helsinki Tukholmankatu 8, FIN\u201000290 Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppa","family":"Mud\u00f2","sequence":"additional","affiliation":[{"name":"Department of Experimental Biomedicine and Clinical Neuroscience Division of Human Physiology University of Palermo Corso Tukory 129, I\u201090134 Palermo Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan Duc","family":"Pham","sequence":"additional","affiliation":[{"name":"Medicum Department of Biochemistry and Developmental Biology Medical Faculty University of Helsinki POB 63, 00014, Haartmaninkatu 8 FIN\u201000290 Helsinki Finland"},{"name":"Minerva Medical Research Institute Biomedicum\u20102 Helsinki Tukholmankatu 8, FIN\u201000290 Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Di Liberto","sequence":"additional","affiliation":[{"name":"Department of Experimental Biomedicine and Clinical Neuroscience Division of Human Physiology University of Palermo Corso Tukory 129, I\u201090134 Palermo Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ove","family":"Eriksson","sequence":"additional","affiliation":[{"name":"Medicum Department of Biochemistry and Developmental Biology Medical Faculty University of Helsinki POB 63, 00014, Haartmaninkatu 8 FIN\u201000290 Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8649-781X","authenticated-orcid":false,"given":"Lauri","family":"Louhivuori","sequence":"additional","affiliation":[{"name":"Medicum Department of Physiology University of Helsinki Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9line","family":"Bruelle","sequence":"additional","affiliation":[{"name":"Medicum Department of Biochemistry and Developmental Biology Medical Faculty University of Helsinki POB 63, 00014, Haartmaninkatu 8 FIN\u201000290 Helsinki Finland"},{"name":"Minerva Medical Research Institute Biomedicum\u20102 Helsinki Tukholmankatu 8, FIN\u201000290 Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rabah","family":"Soliymani","sequence":"additional","affiliation":[{"name":"Medicum Department of Biochemistry and Developmental Biology Medical Faculty University of Helsinki POB 63, 00014, Haartmaninkatu 8 FIN\u201000290 Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Baumann","sequence":"additional","affiliation":[{"name":"Medicum Department of Biochemistry and Developmental Biology Medical Faculty University of Helsinki POB 63, 00014, Haartmaninkatu 8 FIN\u201000290 Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Korhonen","sequence":"additional","affiliation":[{"name":"Medicum Department of Biochemistry and Developmental Biology Medical Faculty University of Helsinki POB 63, 00014, Haartmaninkatu 8 FIN\u201000290 Helsinki Finland"},{"name":"Clinicum Division of Child Psychiatry Helsinki University Central Hospital Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maciej","family":"Lalowski","sequence":"additional","affiliation":[{"name":"Medicum Department of Biochemistry and Developmental Biology Medical Faculty University of Helsinki POB 63, 00014, Haartmaninkatu 8 FIN\u201000290 Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natale","family":"Belluardo","sequence":"additional","affiliation":[{"name":"Department of Experimental Biomedicine and Clinical Neuroscience Division of Human Physiology University of Palermo Corso Tukory 129, I\u201090134 Palermo Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Lindholm","sequence":"additional","affiliation":[{"name":"Medicum Department of Biochemistry and Developmental Biology Medical Faculty University of Helsinki POB 63, 00014, Haartmaninkatu 8 FIN\u201000290 Helsinki Finland"},{"name":"Minerva Medical Research Institute Biomedicum\u20102 Helsinki Tukholmankatu 8, FIN\u201000290 Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,2,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5381.1322"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.113662"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0953-816x.2001.01847.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnbeh.2011.00045"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0270(96)00121-5"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035357"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2008.10.015"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(08)60767-0"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddr618"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048925"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.2002.1155"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7901908"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.09.015"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2004.02040.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/40901"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.341248"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401472"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2006-0037"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2013.00110"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203765"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.09.016"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2013.170"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddu317"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.09.115"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2009.06898.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2010.11.042"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.2000.0935"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.2003.02826.x"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2004.08.015"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2005.08.004"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05292"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200409171"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.06.138"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-012-1043-0"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2014.0209"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/2193-1801-3-2"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1385\/NMM:3:2:65"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4837"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1180"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-011-0850-z"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.02.035"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(74)90640-4"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2002-0012"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2011.07858.x"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-010-0305-y"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021746"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2012.38"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(96)01387-X"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2015.03.038"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4289-06.2007"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/dds177"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M301990200"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.09.024"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-7431(03)00013-7"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2004.01.014"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2006.10.004"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2006.02.021"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddq163"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3001059"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2007.05948.x"}],"container-title":["European Journal of Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fejn.13157","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejn.13157","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T06:09:38Z","timestamp":1696226978000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ejn.13157"}},"subtitle":[],"editor":[{"given":"Deniz","family":"Kirik","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,2,11]]},"references-count":60,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016,3]]}},"alternative-id":["10.1111\/ejn.13157"],"URL":"https:\/\/doi.org\/10.1111\/ejn.13157","archive":["Portico"],"relation":{},"ISSN":["0953-816X","1460-9568"],"issn-type":[{"type":"print","value":"0953-816X"},{"type":"electronic","value":"1460-9568"}],"subject":[],"published":{"date-parts":[[2016,2,11]]},"assertion":[{"value":"2015-05-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-12-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-02-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}