{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T13:50:37Z","timestamp":1790430637273,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,2,3]],"date-time":"2016-02-03T00:00:00Z","timestamp":1454457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1K01AG041520"],"award-info":[{"award-number":["1K01AG041520"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NS052287"],"award-info":[{"award-number":["NS052287"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NS084477"],"award-info":[{"award-number":["NS084477"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NS079940"],"award-info":[{"award-number":["NS079940"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL091363"],"award-info":[{"award-number":["HL091363"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Narcolepsy is a chronic sleep disorder linked to the loss of orexin\u2010producing neurons in the hypothalamus. Cataplexy, a sudden loss of muscle tone during waking, is an important distinguishing symptom of narcolepsy and it is often triggered by strong emotions. The neural circuit underlying cataplexy attacks is not known, but is likely to involve the amygdala, a region implicated in regulating emotions. In mice models of narcolepsy, transfer of the orexin gene into surrogate neurons has been successful in ameliorating narcoleptic symptoms. However, it is not known whether this method also blocks cataplexy triggered by strong emotions. To examine this possibility, the gene encoding mouse prepro\u2010orexin was transferred into amygdala neurons of orexin\u2010knockout (<jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content>) mice (<jats:styled-content style=\"fixed-case\">rAAV<\/jats:styled-content>\u2010orexin; <jats:italic>n<\/jats:italic>\u00a0=\u00a08). Orexin\u2010<jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content> mice that did not receive gene transfer (no\u2010<jats:styled-content style=\"fixed-case\">rAAV<\/jats:styled-content>;<jats:italic> n<\/jats:italic>\u00a0=\u00a07) or received only the reporter gene (<jats:styled-content style=\"fixed-case\">rAAV<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content>;<jats:italic> n<\/jats:italic>\u00a0=\u00a07) served as controls. Three weeks later, the animal's sleep and behaviour were recorded at night (no\u2010odour control night), followed by another recording at night in the presence of predator odour (odour night). Orexin\u2010<jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content> mice given the orexin gene transfer into surrogate amygdala neurons had significantly less spontaneous bouts of cataplexy, and predator odour did not induce cataplexy compared with control mice. Moreover, the mice with orexin gene transfer were awake more during the odour night. These results demonstrate that orexin gene transfer into amygdala neurons can suppress both spontaneous and emotion\u2010induced cataplexy attacks in narcoleptic mice. It suggests that manipulating amygdala pathways is a potential strategy for treating cataplexy in narcolepsy.<\/jats:p>","DOI":"10.1111\/ejn.13158","type":"journal-article","created":{"date-parts":[[2016,1,7]],"date-time":"2016-01-07T13:32:47Z","timestamp":1452173567000},"page":"681-688","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Orexin gene transfer into the amygdala suppresses both spontaneous and emotion\u2010induced cataplexy in orexin\u2010knockout mice"],"prefix":"10.1111","volume":"43","author":[{"given":"Meng","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Psychiatry and Behavioral Sciences Medical University of South Carolina Charleston SC 29425 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Blanco\u2010Centurion","sequence":"additional","affiliation":[{"name":"Department of Psychiatry and Behavioral Sciences Medical University of South Carolina Charleston SC 29425 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roda Rani","family":"Konadhode","sequence":"additional","affiliation":[{"name":"Department of Psychiatry and Behavioral Sciences Medical University of South Carolina Charleston SC 29425 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liju","family":"Luan","sequence":"additional","affiliation":[{"name":"Department of Psychiatry and Behavioral Sciences Medical University of South Carolina Charleston SC 29425 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priyattam J.","family":"Shiromani","sequence":"additional","affiliation":[{"name":"Department of Psychiatry and Behavioral Sciences Medical University of South Carolina Charleston SC 29425 USA"},{"name":"Ralph H. Johnson VA Medical Center Medical University of South Carolina Charleston SC 29425 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,2,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0301-0082(93)90042-Q"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2006.07.018"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.5665\/sleep.2296"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.2003.02861.x"},{"key":"e_1_2_7_6_1","first-page":"652","article-title":"Volume of the amygdala is reduced in patients with narcolepsy \u2013 a structural MRI study","volume":"32","author":"Brabec J.","year":"2011","journal-title":"Neuroendocrinol. Lett."},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5632-12.2013"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2010.12.001"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81973-X"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-0115(01)00357-3"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/sleep\/30.11.1417"},{"key":"e_1_2_7_12_1","first-page":"123","article-title":"REM sleep at its core \u2013 circuits, neurotransmitters, and pathophysiology","volume":"29","author":"Fraigne J.J.","year":"2015","journal-title":"Front. Neurol."},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.22554"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(02)00089-1"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(01)00293-8"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI71017"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0811400106"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2011.04.025"},{"key":"e_1_2_7_19_1","volume-title":"Experimental Design: Procedures for the Behavioral Sciences","author":"Kirk R.E.","year":"1968"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81965-0"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2008.06446.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.6069-10.2011"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.1190"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sleep.2007.05.006"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0840-15.2015"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2011.04.005"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)05582-8"},{"key":"e_1_2_7_28_1","volume-title":"The Mouse Brain in Stereotaxic Coordinates","author":"Paxinos G.","year":"2013"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/79690"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2005.09.025"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.5665\/sleep\/32.1.111"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1925546"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)00058-1"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-010-9437-7"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2012.08.022"}],"container-title":["European Journal of Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fejn.13158","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejn.13158","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T06:09:18Z","timestamp":1696226958000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ejn.13158"}},"subtitle":[],"editor":[{"given":"Rae","family":"Silver","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,2,3]]},"references-count":34,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016,3]]}},"alternative-id":["10.1111\/ejn.13158"],"URL":"https:\/\/doi.org\/10.1111\/ejn.13158","archive":["Portico"],"relation":{},"ISSN":["0953-816X","1460-9568"],"issn-type":[{"value":"0953-816X","type":"print"},{"value":"1460-9568","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,2,3]]},"assertion":[{"value":"2015-10-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-12-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-02-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}