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Here, we used single\u2010cell quantitative polymerase chain reaction to assess co\u2010expression of<jats:styled-content style=\"fixed-case\">AMY<\/jats:styled-content>subunits in<jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>neurons from rats that were injected with amylin or vehicle. Because amylin interacts synergistically with the adipokine leptin to reduce body weight, we also assessed the co\u2010expression of<jats:styled-content style=\"fixed-case\">AMY<\/jats:styled-content>and the leptin receptor isoform b (LepRb) in amylin\u2010activated<jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>neurons. Single cells were collected from Wistar rats and from transgenic<jats:italic>Fos\u2010<jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content><\/jats:italic>rats that express green fluorescent protein (<jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content>) under the control of the Fos promoter. We found that the<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s of<jats:styled-content style=\"fixed-case\">CTR<\/jats:styled-content>a,<jats:styled-content style=\"fixed-case\">RAMP<\/jats:styled-content>1,<jats:styled-content style=\"fixed-case\">RAMP<\/jats:styled-content>2 and<jats:styled-content style=\"fixed-case\">RAMP<\/jats:styled-content>3 were all co\u2010expressed in single<jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>neurons. Moreover, most of the<jats:styled-content style=\"fixed-case\">CTR<\/jats:styled-content>a+ cells co\u2010expressed more than one of the<jats:styled-content style=\"fixed-case\">RAMP<\/jats:styled-content>s. Amylin down\u2010regulated<jats:styled-content style=\"fixed-case\">RAMP<\/jats:styled-content>1 and<jats:styled-content style=\"fixed-case\">RAMP<\/jats:styled-content>3 but not<jats:styled-content style=\"fixed-case\">CTR mRNA<\/jats:styled-content>s in<jats:styled-content style=\"fixed-case\">AMY<\/jats:styled-content>+ neurons, suggesting a possible negative feedback mechanism of amylin at its own primary receptors. Interestingly, amylin up\u2010regulated<jats:styled-content style=\"fixed-case\">RAMP<\/jats:styled-content>2<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>. We also found that a high percentage of single cells that co\u2010expressed all components of a functional<jats:styled-content style=\"fixed-case\">AMY<\/jats:styled-content>expressed LepRb<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>. Thus, single<jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>cells expressed both<jats:styled-content style=\"fixed-case\">AMY<\/jats:styled-content>and LepRb, which formed a population of first\u2010order neurons that presumably can be directly activated by amylin and, at least in part, also by leptin.<\/jats:p>","DOI":"10.1111\/ejn.13163","type":"journal-article","created":{"date-parts":[[2016,1,11]],"date-time":"2016-01-11T02:55:19Z","timestamp":1452480919000},"page":"653-661","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":71,"title":["Amylin receptor components and the leptin receptor are co\u2010expressed in single rat area postrema neurons"],"prefix":"10.1111","volume":"43","author":[{"given":"Claudia G.","family":"Liberini","sequence":"first","affiliation":[{"name":"Institute of Veterinary Physiology Vetsuisse Faculty University of Z\u00fcrich (UZH) Winterthurerstrasse 260 CH\u20108057 Z\u00fcrich 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