{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T09:44:36Z","timestamp":1775727876585,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,6,8]],"date-time":"2017-06-08T00:00:00Z","timestamp":1496880000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001602","name":"Science Foundation Ireland","doi-asserted-by":"publisher","award":["13\/IA\/1783"],"award-info":[{"award-number":["13\/IA\/1783"]}],"id":[{"id":"10.13039\/501100001602","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Retinitis pigmentosa (<jats:styled-content style=\"fixed-case\">RP<\/jats:styled-content>) is a group of hereditary retinal diseases, characterised by photoreceptor cell loss. Despite a substantial understanding of the mechanisms leading to cell death, an effective therapeutic strategy is sought. Our laboratory has previously demonstrated the neuroprotective properties of Norgestrel, a progesterone analogue, in the degenerating retina, mediated in part by the neurotrophic factor basic fibroblast growth factor (<jats:styled-content style=\"fixed-case\">bFGF<\/jats:styled-content>). In other retinal studies, we have also presented a pro\u2010survival role for reactive oxygen species (<jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>), downstream of <jats:styled-content style=\"fixed-case\">bFGF<\/jats:styled-content>. Thus, we hypothesized that Norgestrel utilises <jats:styled-content style=\"fixed-case\">bFGF<\/jats:styled-content>\u2010driven <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> production to promote photoreceptor survival. Using the 661W photoreceptor\u2010like cell line, we now show that Norgestrel, working through progesterone receptor membrane complex 1 (<jats:styled-content style=\"fixed-case\">PGRMC<\/jats:styled-content>1); generates an early burst of pro\u2010survival <jats:styled-content style=\"fixed-case\">bFGF<\/jats:styled-content>\u2010induced <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>. Using the rd10 mouse model of <jats:styled-content style=\"fixed-case\">RP<\/jats:styled-content>, we confirm that Norgestrel induces a similar early pro\u2010survival increase in retinal <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>. Norgestrel\u2010driven protection in the rd10 retina was attenuated in the presence of antioxidants. This study therefore presents an essential role for <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> signalling in Norgestrel\u2010mediated neuroprotection <jats:italic>in\u00a0vitro<\/jats:italic> and demonstrates that Norgestrel employs a similar pro\u2010survival mechanism in the degenerating retina.<\/jats:p>","DOI":"10.1111\/ejn.13604","type":"journal-article","created":{"date-parts":[[2017,5,11]],"date-time":"2017-05-11T19:41:34Z","timestamp":1494531694000},"page":"1663-1672","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Pro\u2010survival redox signalling in progesterone\u2010mediated retinal neuroprotection"],"prefix":"10.1111","volume":"46","author":[{"given":"Ana M.","family":"Ruiz Lopez","sequence":"first","affiliation":[{"name":"Cell Development and Disease Laboratory Biochemistry Department Biosciences Institute University College Cork Cork Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah L.","family":"Roche","sequence":"additional","affiliation":[{"name":"Cell Development and Disease Laboratory Biochemistry Department Biosciences Institute University College Cork Cork Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice C.","family":"Wyse Jackson","sequence":"additional","affiliation":[{"name":"Cell Development and Disease Laboratory Biochemistry Department Biosciences Institute University College Cork Cork Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer N.","family":"Moloney","sequence":"additional","affiliation":[{"name":"Cell Development and Disease Laboratory Biochemistry Department Biosciences Institute University College Cork Cork Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashleigh M.","family":"Byrne","sequence":"additional","affiliation":[{"name":"Cell Development and Disease Laboratory Biochemistry Department Biosciences Institute University College Cork Cork Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas G.","family":"Cotter","sequence":"additional","affiliation":[{"name":"Cell Development and Disease Laboratory Biochemistry Department Biosciences Institute University College Cork Cork Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,6,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2008.06.042"},{"key":"e_1_2_10_3_1","first-page":"283","article-title":"Rod and cone photoreceptor cells produce ROS in response to stress in a live retinal explant system","volume":"16","author":"Bhatt L.","year":"2010","journal-title":"Mol. 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